Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne Bearing Noncanonical Amino Acids

Kurzanzeige
dc.contributor.authorBraun, Theresa S.
dc.contributor.authorWidder, Pia
dc.contributor.authorOsswald, Uwe
dc.contributor.authorGroß, Lina
dc.contributor.authorWilliams, Lara
dc.contributor.authorSchmidt, Moritz J.
dc.contributor.authorHelmle, Irina
dc.contributor.authorSummerer, Daniel
dc.contributor.authorDrescher, Malte
dc.date.accessioned2019-11-13T10:00:26Z
dc.date.available2019-11-13T10:00:26Z
dc.date.issued2020-04
dc.description.abstractElectron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling (SDSL) is a powerful tool in protein structural research. Nitroxides are highly suitable spin labeling reagents, but suffer from limited stability in particular in cellular environment. Here, we present the synthesis of a maleimid- and an azide-modified tetraethyl-shielded isoindoline-based nitroxide (M- and Az-TEIO) for labeling of cysteines or the noncanonical amino acid para -ethynyl-l-phenylalanine ( p ENF). We demonstrate high stability of TEIO  site-specifically attached to the protein thioredoxin (TRX) towards reduction in prokaryotic and eukaryotic environments, and conduct double electron-electron resonance (DEER) measurements. We further generate a rotamer library for the new residue p ENF-Az-TEIO that affords a distance distribution that is in agreement with the measured distribution.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1002/cbic.201900537eng
dc.identifier.pmid31657498eng
dc.identifier.ppn1697302238
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/47475
dc.language.isoengeng
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectbioresistancy, EPR spectroscopy, macromolecular dynamics, protein conformation analysis, site-directed spin labelingeng
dc.subject.ddc540eng
dc.titleIsoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne Bearing Noncanonical Amino Acidseng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Braun2020-04Isoin-47475,
  year={2020},
  doi={10.1002/cbic.201900537},
  title={Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne Bearing Noncanonical Amino Acids},
  number={7},
  volume={21},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={958--962},
  author={Braun, Theresa S. and Widder, Pia and Osswald, Uwe and Groß, Lina and Williams, Lara and Schmidt, Moritz J. and Helmle, Irina and Summerer, Daniel and Drescher, Malte}
}
kops.citation.iso690BRAUN, Theresa S., Pia WIDDER, Uwe OSSWALD, Lina GROSS, Lara WILLIAMS, Moritz J. SCHMIDT, Irina HELMLE, Daniel SUMMERER, Malte DRESCHER, 2020. Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne Bearing Noncanonical Amino Acids. In: ChemBioChem. Wiley. 2020, 21(7), pp. 958-962. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201900537deu
kops.citation.iso690BRAUN, Theresa S., Pia WIDDER, Uwe OSSWALD, Lina GROSS, Lara WILLIAMS, Moritz J. SCHMIDT, Irina HELMLE, Daniel SUMMERER, Malte DRESCHER, 2020. Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne Bearing Noncanonical Amino Acids. In: ChemBioChem. Wiley. 2020, 21(7), pp. 958-962. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201900537eng
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