Publikation:

Exploiting the multiplexing capabilities of tandem mass tags for high-throughput estimation of cellular protein abundances by mass spectrometry

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Ahrne_2-1c59oncsqlsy58.pdf
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Datum

2015

Autor:innen

Ahrné, Erik
Martinez-Segura, Amalia
Syed, Afzal Pasha
Vina-Vilaseca, Arnau
Marguerat, Samuel

Herausgeber:innen

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Bibliografische Daten

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Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-2-1c59oncsqlsy58
DOI (zitierfähiger Link)
https://doi.org/10.1016/j.ymeth.2015.04.032
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Internationale Patentnummer

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Urheberrechtlich geschützt

Angaben zur Forschungsförderung

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Open Access-Veröffentlichung
Open Access Green

Sammlungen

Biologie: Publikationen
Core Facility der Universität Konstanz

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Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Methods. Elsevier. 2015, 85, pp. 100-107. ISSN 1046-2023. eISSN 1095-9130. Available under: doi: 10.1016/j.ymeth.2015.04.032

Zusammenfassung

The generation of dynamic models of biological processes critically depends on the determination of precise cellular concentrations of biomolecules. Measurements of system-wide absolute protein levels are particularly valuable information in systems biology. Recently, mass spectrometry based proteomics approaches have been developed to estimate protein concentrations on a proteome-wide scale. However, for very complex proteomes, fractionation steps are required, increasing samples number and instrument analysis time. As a result, the number of full proteomes that can be routinely analyzed is limited. Here we combined absolute quantification strategies with the multiplexing capabilities of isobaric tandem mass tags to determine cellular protein abundances in a high throughput and proteome-wide scale even for highly complex biological systems, such as a whole human cell line. We generated two independent data sets to demonstrate the power of the approach regarding sample throughput, dynamic range, quantitative precision and accuracy as well as proteome coverage in comparison to existing mass spectrometry based strategies.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Mass spectrometry, Absolute protein quantification, Tandem mass tags, Schizosaccharomyces pombe, HEK 293, iBAQ

Konferenz

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ISO 690AHRNÉ, Erik, Amalia MARTINEZ-SEGURA, Afzal Pasha SYED, Arnau VINA-VILASECA, Andreas J. GRUBER, Samuel MARGUERAT, Alexander SCHMIDT, 2015. Exploiting the multiplexing capabilities of tandem mass tags for high-throughput estimation of cellular protein abundances by mass spectrometry. In: Methods. Elsevier. 2015, 85, pp. 100-107. ISSN 1046-2023. eISSN 1095-9130. Available under: doi: 10.1016/j.ymeth.2015.04.032
BibTex
@article{Ahrne2015-09-01Explo-50907,
  year={2015},
  doi={10.1016/j.ymeth.2015.04.032},
  title={Exploiting the multiplexing capabilities of tandem mass tags for high-throughput estimation of cellular protein abundances by mass spectrometry},
  volume={85},
  issn={1046-2023},
  journal={Methods},
  pages={100--107},
  author={Ahrné, Erik and Martinez-Segura, Amalia and Syed, Afzal Pasha and Vina-Vilaseca, Arnau and Gruber, Andreas J. and Marguerat, Samuel and Schmidt, Alexander}
}
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Interner Vermerk

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