Publikation:

Accumulation of potentiometric and other dyes in haustoria of Erysiphe graminis in living host cells

Lade...
Vorschaubild

Datum

1987

Autor:innen

Bushnell, William R.
Liu, Z.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Physiological and Molecular Plant Pathology. 1987, 31(2), pp. 237-250. ISSN 0885-5765. Available under: doi: 10.1016/0885-5765(87)90068-3

Zusammenfassung

Twenty-five vital fluorescent dyes were tested for accumulation in haustoria of Erysiphe graminis (DC) Merat f. sp. hordei Em. Marchal when applied to coleoptile epidermal host cells of Hordeum vulgare L. Eight of the dyes gave fluorescence to structures judged to be haustorial mitochondria. The potentiometric cyanine dyes, DiOC4(3) and DiOC7(3) gave best differentiation between the putative mitochondria and other structures. As measured photometrically, fluorescence of the organelles diminished within a few minutes after DNP(1 × 10−4M), CCCP (1 × 10−6M), or KCN (1 × 10−3M) was applied to host tissue, indicating that fluorescence depended on the membrane potential of the organelles.

Many of the tested dyes, including several cyanines, accumulated in haustorial cytoplasm (excluding mitochondria or vacuoles), but the uptake of most of these dyes was not affected by DNP or CCCP, indicating that the dyes moved passively into haustoria. However the uptake of the cyanines DiOC5(3) and DiSC2(3) was slightly reduced by CCCP suggesting that a component of uptake of these dyes depended on the potential of the plasmalemma or extrahaustorial membrane of the haustorium.

The cyanine potentiometric dyes provide a way to monitor electrical potential across mitochondrial and other membranes which, in turn, may relate to functional competence of haustoria.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690BUSHNELL, William R., Kurt MENDGEN, Z. LIU, 1987. Accumulation of potentiometric and other dyes in haustoria of Erysiphe graminis in living host cells. In: Physiological and Molecular Plant Pathology. 1987, 31(2), pp. 237-250. ISSN 0885-5765. Available under: doi: 10.1016/0885-5765(87)90068-3
BibTex
@article{Bushnell1987Accum-7667,
  year={1987},
  doi={10.1016/0885-5765(87)90068-3},
  title={Accumulation of potentiometric and other dyes in haustoria of Erysiphe graminis in living host cells},
  number={2},
  volume={31},
  issn={0885-5765},
  journal={Physiological and Molecular Plant Pathology},
  pages={237--250},
  author={Bushnell, William R. and Mendgen, Kurt and Liu, Z.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7667">
    <dc:contributor>Liu, Z.</dc:contributor>
    <dcterms:issued>1987</dcterms:issued>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:36:13Z</dcterms:available>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7667/1/Accumulation_of_potentiometric_and_other_dyes_in_haustoria_of_Erysiphe_graminis_in_living_host_cells.pdf"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:language>eng</dc:language>
    <dcterms:title>Accumulation of potentiometric and other dyes in haustoria of Erysiphe graminis in living host cells</dcterms:title>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7667"/>
    <dc:contributor>Mendgen, Kurt</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dc:creator>Liu, Z.</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7667/1/Accumulation_of_potentiometric_and_other_dyes_in_haustoria_of_Erysiphe_graminis_in_living_host_cells.pdf"/>
    <dc:creator>Bushnell, William R.</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Physiological and Molecular Plant Pathology 31 (1987), pp. 237-250</dcterms:bibliographicCitation>
    <dcterms:abstract xml:lang="eng">Twenty-five vital fluorescent dyes were tested for accumulation in haustoria of Erysiphe graminis (DC) Merat f. sp. hordei Em. Marchal when applied to coleoptile epidermal host cells of Hordeum vulgare L. Eight of the dyes gave fluorescence to structures judged to be haustorial mitochondria. The potentiometric cyanine dyes, DiOC4(3) and DiOC7(3) gave best differentiation between the putative mitochondria and other structures. As measured photometrically, fluorescence of the organelles diminished within a few minutes after DNP(1 × 10−4M), CCCP (1 × 10−6M), or KCN (1 × 10−3M) was applied to host tissue, indicating that fluorescence depended on the membrane potential of the organelles.&lt;br /&gt;&lt;br /&gt;Many of the tested dyes, including several cyanines, accumulated in haustorial cytoplasm (excluding mitochondria or vacuoles), but the uptake of most of these dyes was not affected by DNP or CCCP, indicating that the dyes moved passively into haustoria. However the uptake of the cyanines DiOC5(3) and DiSC2(3) was slightly reduced by CCCP suggesting that a component of uptake of these dyes depended on the potential of the plasmalemma or extrahaustorial membrane of the haustorium.&lt;br /&gt;&lt;br /&gt;The cyanine potentiometric dyes provide a way to monitor electrical potential across mitochondrial and other membranes which, in turn, may relate to functional competence of haustoria.</dcterms:abstract>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:creator>Mendgen, Kurt</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:36:13Z</dc:date>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dc:contributor>Bushnell, William R.</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen