Site-specific labeling of proteins with NMR-active unnatural amino acids

Kurzanzeige
dc.contributor.authorJones, David H.deu
dc.contributor.authorCellitti, Susan E.deu
dc.contributor.authorHao, Xueshideu
dc.contributor.authorZhang, Qiongdeu
dc.contributor.authorJahnz, Michaeldeu
dc.contributor.authorSummerer, Daniel
dc.contributor.authorSchultz, Peter G.deu
dc.contributor.authorUno, Tetsuodeu
dc.contributor.authorGeierstanger, Bernhard H.deu
dc.date.accessioned2011-11-11T09:04:54Zdeu
dc.date.available2011-11-11T09:04:54Zdeu
dc.date.issued2010-01
dc.description.abstractA large number of amino acids other than the canonical amino acids can now be easily incorporated in vivo into proteins at genetically encoded positions. The technology requires an orthogonal tRNA/aminoacyl-tRNA synthetase pair specific for the unnatural amino acid that is added to the media while a TAG amber or frame shift codon specifies the incorporation site in the protein to be studied. These unnatural amino acids can be isotopically labeled and provide unique opportunities for site-specific labeling of proteins for NMR studies. In this perspective, we discuss these opportunities including new photocaged unnatural amino acids, outline usage of metal chelating and spin-labeled unnatural amino acids and expand the approach to in-cell NMR experiments.eng
dc.description.versionpublished
dc.identifier.citationFirst publ. in: Journal of Biomolecular NMR ; 46 (2010), 1. - S. 89-100deu
dc.identifier.doi10.1007/s10858-009-9365-4deu
dc.identifier.pmid19669620
dc.identifier.ppn352980613deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/13747
dc.language.isoengdeu
dc.legacy.dateIssued2011-11-11deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subjectSite-specific labelingdeu
dc.subjectunnatural amino acidsdeu
dc.subjectspin labeldeu
dc.subjectmetal chelatordeu
dc.subjectin-cell NMRdeu
dc.subject.ddc540deu
dc.titleSite-specific labeling of proteins with NMR-active unnatural amino acidseng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Jones2010-01Sites-13747,
  year={2010},
  doi={10.1007/s10858-009-9365-4},
  title={Site-specific labeling of proteins with NMR-active unnatural amino acids},
  number={1},
  volume={46},
  issn={0925-2738},
  journal={Journal of Biomolecular NMR},
  pages={89--100},
  author={Jones, David H. and Cellitti, Susan E. and Hao, Xueshi and Zhang, Qiong and Jahnz, Michael and Summerer, Daniel and Schultz, Peter G. and Uno, Tetsuo and Geierstanger, Bernhard H.}
}
kops.citation.iso690JONES, David H., Susan E. CELLITTI, Xueshi HAO, Qiong ZHANG, Michael JAHNZ, Daniel SUMMERER, Peter G. SCHULTZ, Tetsuo UNO, Bernhard H. GEIERSTANGER, 2010. Site-specific labeling of proteins with NMR-active unnatural amino acids. In: Journal of Biomolecular NMR. 2010, 46(1), pp. 89-100. ISSN 0925-2738. eISSN 1573-5001. Available under: doi: 10.1007/s10858-009-9365-4deu
kops.citation.iso690JONES, David H., Susan E. CELLITTI, Xueshi HAO, Qiong ZHANG, Michael JAHNZ, Daniel SUMMERER, Peter G. SCHULTZ, Tetsuo UNO, Bernhard H. GEIERSTANGER, 2010. Site-specific labeling of proteins with NMR-active unnatural amino acids. In: Journal of Biomolecular NMR. 2010, 46(1), pp. 89-100. ISSN 0925-2738. eISSN 1573-5001. Available under: doi: 10.1007/s10858-009-9365-4eng
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kops.sourcefieldJournal of Biomolecular NMR. 2010, <b>46</b>(1), pp. 89-100. ISSN 0925-2738. eISSN 1573-5001. Available under: doi: 10.1007/s10858-009-9365-4deu
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kops.sourcefield.plainJournal of Biomolecular NMR. 2010, 46(1), pp. 89-100. ISSN 0925-2738. eISSN 1573-5001. Available under: doi: 10.1007/s10858-009-9365-4eng
kops.submitter.emailoleg.kozlov@uni-konstanz.dedeu
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temp.target.additionalFachbereich Chemiedeu

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