Publikation: RNA unwinding activity of SV40 large T antigen
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Large T antigen, the regulatory protein encoded by simian virus 40, has DNA helicase activity and unwinds double-stranded DNA at the expense of ATP. T antigen also functions as an RNA helicase separating duplex regions in partially double-stranded RNA substrates. Surprisingly, T antigen RNA helicase activity requires UTP, CTP, or GTP as a cofactor, whereas ATP is an inefficient energy source for the RNA unwinding reaction. Accordingly, T antigen has both an intrinsic non-ATP NTPase activity that is stimulated by single-stranded RNA and an ATPase activity stimulated by single-stranded DNA. Thus, it appears that the bound nucleotide determines whether T antigen acts as an RNA helicase or as a DNA helicase.
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SCHEFFNER, Martin, Rolf KNIPPERS, Hans STAHL, 1989. RNA unwinding activity of SV40 large T antigen. In: Cell. 1989, 57(6), pp. 955-963. ISSN 0092-8674. eISSN 1097-4172. Available under: doi: 10.1016/0092-8674(89)90334-6BibTex
@article{Scheffner1989-06unwin-42736, year={1989}, doi={10.1016/0092-8674(89)90334-6}, title={RNA unwinding activity of SV40 large T antigen}, number={6}, volume={57}, issn={0092-8674}, journal={Cell}, pages={955--963}, author={Scheffner, Martin and Knippers, Rolf and Stahl, Hans} }
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