Publikation: Deficient IFN-gamma Expression in Umbilical Cord Blood (UCB) T Cells Can Be Rescued by IFN-gamma-Mediated Increase in NFATc2 Expression
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Regulation of nuclear factor of activated T cells-c2 (NFATc2) gene expression is not clearly defined. We previously reported reduced NFATc2 protein expression in cord blood T lymphocytes. Here we show that NFATc2 expression in T cells is dependent in part on the presence of IFN-gamma during primary stimulation, as blocking of IFN-gamma blunted NFATc2 protein and mRNA upregulation. Conversely, addition of exogenous IFN-gamma during stimulation resulted in increased expression of NFATc2 in cord blood T lymphocytes. This correlated with rescue of deficient IFN-gamma expression by cord blood T cells. Rescue of IFN-gamma expression in cord blood T cells was dependent on the presence of antigen-presenting cells, as addition of IFN-gamma during stimulation of purified cord blood T cells did not result in an increase of IFN-gamma expression, and depletion of monocytes ablated the rescue of IFN-gamma expression. Our results point to impaired function in the antigen-presenting cell population of cord blood, playing a role in the hyporesponsiveness of T cells.
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KADEREIT, Suzanne, Gwendolyn R. JUNGE, Thomas KLEEN, Margaret M. KOZIK, Beth A. KAMINSKI, Kathleen DAUM-WOODS, Pingfu FU, Magdalena TARY-LEHMANN, Mary J. LAUGHLIN, 2003. Deficient IFN-gamma Expression in Umbilical Cord Blood (UCB) T Cells Can Be Rescued by IFN-gamma-Mediated Increase in NFATc2 Expression. In: Journal of Clinical Immunology. 2003, 23(6), pp. 485-497. eISSN 0271-9142. Available under: doi: 10.1023/B:JOCI.0000010425.30967.0fBibTex
@article{Kadereit2003Defic-7661, year={2003}, doi={10.1023/B:JOCI.0000010425.30967.0f}, title={Deficient IFN-gamma Expression in Umbilical Cord Blood (UCB) T Cells Can Be Rescued by IFN-gamma-Mediated Increase in NFATc2 Expression}, number={6}, volume={23}, journal={Journal of Clinical Immunology}, pages={485--497}, author={Kadereit, Suzanne and Junge, Gwendolyn R. and Kleen, Thomas and Kozik, Margaret M. and Kaminski, Beth A. and Daum-Woods, Kathleen and Fu, Pingfu and Tary-Lehmann, Magdalena and Laughlin, Mary J.} }
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