Crystallization and preliminary X-ray diffraction analysis of ScrY, a specific bacterial outer membrane porin

Kurzanzeige
dc.contributor.authorForst, Dorisdeu
dc.contributor.authorSchülein, Katrindeu
dc.contributor.authorWacker, Thomasdeu
dc.contributor.authorDiederichs, Kay
dc.contributor.authorKreutz, Wernerdeu
dc.contributor.authorBenz, Rolanddeu
dc.contributor.authorWelte, Wolfram
dc.date.accessioned2011-03-24T17:42:32Zdeu
dc.date.available2011-03-24T17:42:32Zdeu
dc.date.issued1993deu
dc.description.abstractThe sucrose-specific outer membrane porin ScrY of Salmonella typhimurium was isolated from Escherichia coli K-12 strain KS 26 containing the plasmid pPSO112. The protein was purified to homogeneity by differential extraction of the cell envelope in the presence of the detergents sodium dodecyl sulfate and lauryl (dimethyl)-amine oxide (LDAO). The porin had apparent molecular weights of 58 kDa and 120 kDa for the monomer and for the trimer, respectively, on SDS/PAGE. The purified trimers were crystallized using poly(ethylene glycol) 2000 and the detergents octylglucoside (OG) and hexyl-(dimethyl)-amine oxide (C6DAO). X-ray diffraction of the crystals showed reflections to 2'3 Å. The space group of the crystals was R3 and the lattice constants of the hexagonal axes were a = b = 112'85 Å and c =149'9 Å. The crystal volume per unit of protein molecular weight was 3'47 Å3/Da.deu
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Journal of Molecular Biology 229 (1993), pp. 258-262deu
dc.identifier.doi10.1006/jmbi.1993.1028
dc.identifier.pmid8421311
dc.identifier.ppn273742264deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/8304
dc.language.isoengdeu
dc.legacy.dateIssued2007deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subjectporindeu
dc.subjectsucrose transportdeu
dc.subjectmembrane proteindeu
dc.subjectcrystallizationdeu
dc.subjectE. colideu
dc.subject.ddc570deu
dc.titleCrystallization and preliminary X-ray diffraction analysis of ScrY, a specific bacterial outer membrane porineng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Forst1993Cryst-8304,
  year={1993},
  doi={10.1006/jmbi.1993.1028},
  title={Crystallization and preliminary X-ray diffraction analysis of ScrY, a specific bacterial outer membrane porin},
  number={1},
  volume={229},
  issn={0022-2836},
  journal={Journal of Molecular Biology},
  pages={258--262},
  author={Forst, Doris and Schülein, Katrin and Wacker, Thomas and Diederichs, Kay and Kreutz, Werner and Benz, Roland and Welte, Wolfram}
}
kops.citation.iso690FORST, Doris, Katrin SCHÜLEIN, Thomas WACKER, Kay DIEDERICHS, Werner KREUTZ, Roland BENZ, Wolfram WELTE, 1993. Crystallization and preliminary X-ray diffraction analysis of ScrY, a specific bacterial outer membrane porin. In: Journal of Molecular Biology. 1993, 229(1), pp. 258-262. ISSN 0022-2836. Available under: doi: 10.1006/jmbi.1993.1028deu
kops.citation.iso690FORST, Doris, Katrin SCHÜLEIN, Thomas WACKER, Kay DIEDERICHS, Werner KREUTZ, Roland BENZ, Wolfram WELTE, 1993. Crystallization and preliminary X-ray diffraction analysis of ScrY, a specific bacterial outer membrane porin. In: Journal of Molecular Biology. 1993, 229(1), pp. 258-262. ISSN 0022-2836. Available under: doi: 10.1006/jmbi.1993.1028eng
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