Assessment of pyrogenic contaminations with validated human whole-blood assay

Kurzanzeige
dc.contributor.authorDaneshian, Mardas
dc.contributor.authorAulock, Sonja von
dc.contributor.authorHartung, Thomas
dc.date.accessioned2011-03-24T17:05:29Zdeu
dc.date.available2011-03-24T17:05:29Zdeu
dc.date.issued2009deu
dc.description.abstractWe present an internationally validated protocol for the evaluation of pyrogenic contaminations using human whole blood. In the in vitro pyrogen test (IPT) the sample is incubated with fresh or cryopreserved human whole blood, and the proinflammatory cytokine interleukin-1 beta (IL-1 beta) is detected by enzyme-linked immunosorbent assay (ELISA). In addition to detecting pyrogenic contaminations in aqueous samples, e.g., parenteral drugs; adaptations allow the assessment of lipidic, toxic or immunomodulatory substances; detection of low-grade contaminations in large-volume parenterals, e.g., dialysis water and fluids; pyrogenicity assessment of solid materials, e.g., medical devices; and evaluation of airborne pyrogenic burden. In contrast to the rabbit pyrogen test and the limulus amoebocyte lysate (LAL) test, it requires no components of animal origin. In comparison with the LAL, it also detects nonlipopolysaccharide pyrogens. In comparison with other monocyte activation tests it requires no cell preparation steps or cell culture facilities. The procedure takes 21-35 h to complete.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Nature protocols 4 (2009), 12, pp. 1709-1721deu
dc.identifier.doi10.1038/nprot.2009.159
dc.identifier.ppn337652066deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/6485
dc.language.isoengdeu
dc.legacy.dateIssued2011deu
dc.rightsterms-of-usedeu
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dc.subject.ddc570deu
dc.titleAssessment of pyrogenic contaminations with validated human whole-blood assayeng
dc.typeJOURNAL_ARTICLEdeu
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kops.citation.bibtex
@article{Daneshian2009Asses-6485,
  year={2009},
  doi={10.1038/nprot.2009.159},
  title={Assessment of pyrogenic contaminations with validated human whole-blood assay},
  number={12},
  volume={4},
  journal={Nature protocols},
  pages={1709--1721},
  author={Daneshian, Mardas and Aulock, Sonja von and Hartung, Thomas}
}
kops.citation.iso690DANESHIAN, Mardas, Sonja von AULOCK, Thomas HARTUNG, 2009. Assessment of pyrogenic contaminations with validated human whole-blood assay. In: Nature protocols. 2009, 4(12), pp. 1709-1721. Available under: doi: 10.1038/nprot.2009.159deu
kops.citation.iso690DANESHIAN, Mardas, Sonja von AULOCK, Thomas HARTUNG, 2009. Assessment of pyrogenic contaminations with validated human whole-blood assay. In: Nature protocols. 2009, 4(12), pp. 1709-1721. Available under: doi: 10.1038/nprot.2009.159eng
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kops.sourcefieldNature protocols. 2009, <b>4</b>(12), pp. 1709-1721. Available under: doi: 10.1038/nprot.2009.159deu
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kops.sourcefield.plainNature protocols. 2009, 4(12), pp. 1709-1721. Available under: doi: 10.1038/nprot.2009.159eng
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