Crystallization and calcium/sulfur SAD phasing of the human EF-hand protein S100A2

Kurzanzeige
dc.contributor.authorKoch, Michael
dc.contributor.authorDiez, Joachim
dc.contributor.authorWagner, Armin
dc.contributor.authorFritz, Günter
dc.date.accessioned2021-02-03T12:43:14Z
dc.date.available2021-02-03T12:43:14Z
dc.date.issued2010-09-01eng
dc.description.abstractHuman S100A2 is an EF-hand protein and acts as a major tumour suppressor, binding and activating p53 in a Ca2+-dependent manner. Ca2+-bound S100A2 was crystallized and its structure was determined based on the anomalous scattering provided by six S atoms from methionine residues and four calcium ions present in the asymmetric unit. Although the diffraction data were recorded at a wavelength of 0.90 Å, which is usually not assumed to be suitable for calcium/sulfur SAD, the anomalous signal was satisfactory. A nine-atom substructure was determined at 1.8 Å resolution using SHELXD, and SHELXE was used for density modification and phase extension to 1.3 Å resolution. The electron-density map obtained was well interpretable and could be used for automated model building by ARP/wARP.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1107/S1744309110030691eng
dc.identifier.pmid20823519eng
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/52710
dc.language.isoengeng
dc.subject.ddc570eng
dc.titleCrystallization and calcium/sulfur SAD phasing of the human EF-hand protein S100A2eng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Koch2010-09-01Cryst-52710,
  year={2010},
  doi={10.1107/S1744309110030691},
  title={Crystallization and calcium/sulfur SAD phasing of the human EF-hand protein S100A2},
  number={Pt 9},
  volume={66},
  issn={2053-230X},
  journal={Acta Crystallographica Section F : Structural Biology and Crystallization Communications},
  pages={1032--1036},
  author={Koch, Michael and Diez, Joachim and Wagner, Armin and Fritz, Günter}
}
kops.citation.iso690KOCH, Michael, Joachim DIEZ, Armin WAGNER, Günter FRITZ, 2010. Crystallization and calcium/sulfur SAD phasing of the human EF-hand protein S100A2. In: Acta Crystallographica Section F : Structural Biology and Crystallization Communications. International Union of Crystallography (IUCr). 2010, 66(Pt 9), pp. 1032-1036. ISSN 2053-230X. eISSN 1744-3091. Available under: doi: 10.1107/S1744309110030691deu
kops.citation.iso690KOCH, Michael, Joachim DIEZ, Armin WAGNER, Günter FRITZ, 2010. Crystallization and calcium/sulfur SAD phasing of the human EF-hand protein S100A2. In: Acta Crystallographica Section F : Structural Biology and Crystallization Communications. International Union of Crystallography (IUCr). 2010, 66(Pt 9), pp. 1032-1036. ISSN 2053-230X. eISSN 1744-3091. Available under: doi: 10.1107/S1744309110030691eng
kops.citation.rdf
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52710">
    <dc:contributor>Fritz, Günter</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/52710"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-03T12:43:14Z</dc:date>
    <dc:creator>Koch, Michael</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Diez, Joachim</dc:contributor>
    <dc:contributor>Wagner, Armin</dc:contributor>
    <dc:creator>Fritz, Günter</dc:creator>
    <dc:contributor>Koch, Michael</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Diez, Joachim</dc:creator>
    <dc:creator>Wagner, Armin</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:issued>2010-09-01</dcterms:issued>
    <dcterms:abstract xml:lang="eng">Human S100A2 is an EF-hand protein and acts as a major tumour suppressor, binding and activating p53 in a Ca&lt;sup&gt;2+&lt;/sup&gt;-dependent manner. Ca&lt;sup&gt;2+&lt;/sup&gt;-bound S100A2 was crystallized and its structure was determined based on the anomalous scattering provided by six S atoms from methionine residues and four calcium ions present in the asymmetric unit. Although the diffraction data were recorded at a wavelength of 0.90 Å, which is usually not assumed to be suitable for calcium/sulfur SAD, the anomalous signal was satisfactory. A nine-atom substructure was determined at 1.8 Å resolution using SHELXD, and SHELXE was used for density modification and phase extension to 1.3 Å resolution. The electron-density map obtained was well interpretable and could be used for automated model building by ARP/wARP.</dcterms:abstract>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-03T12:43:14Z</dcterms:available>
    <dcterms:title>Crystallization and calcium/sulfur SAD phasing of the human EF-hand protein S100A2</dcterms:title>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:language>eng</dc:language>
  </rdf:Description>
</rdf:RDF>
kops.flag.isPeerReviewedtrueeng
kops.flag.knbibliographytrue
kops.sourcefieldActa Crystallographica Section F : Structural Biology and Crystallization Communications. International Union of Crystallography (IUCr). 2010, <b>66</b>(Pt 9), pp. 1032-1036. ISSN 2053-230X. eISSN 1744-3091. Available under: doi: 10.1107/S1744309110030691deu
kops.sourcefield.plainActa Crystallographica Section F : Structural Biology and Crystallization Communications. International Union of Crystallography (IUCr). 2010, 66(Pt 9), pp. 1032-1036. ISSN 2053-230X. eISSN 1744-3091. Available under: doi: 10.1107/S1744309110030691deu
kops.sourcefield.plainActa Crystallographica Section F : Structural Biology and Crystallization Communications. International Union of Crystallography (IUCr). 2010, 66(Pt 9), pp. 1032-1036. ISSN 2053-230X. eISSN 1744-3091. Available under: doi: 10.1107/S1744309110030691eng
relation.isAuthorOfPublication3883a6a3-cb82-4992-96ec-30bc101ba570
relation.isAuthorOfPublication5e1e6b8d-ba6b-4303-acaa-278759602ae0
relation.isAuthorOfPublication.latestForDiscovery3883a6a3-cb82-4992-96ec-30bc101ba570
source.bibliographicInfo.fromPage1032eng
source.bibliographicInfo.issuePt 9eng
source.bibliographicInfo.toPage1036eng
source.bibliographicInfo.volume66eng
source.identifier.eissn1744-3091eng
source.identifier.issn2053-230Xeng
source.periodicalTitleActa Crystallographica Section F : Structural Biology and Crystallization Communicationseng
source.publisherInternational Union of Crystallography (IUCr)eng

Dateien

Sammlungen

Biologie: Publikationen