Publikation:

Mapping the helix arrangement of the reconstituted ETR1 ethylene receptor transmembrane domain by EPR spectroscopy

Lade...
Vorschaubild

Dateien

Kugele_2-ivo3pfu89mio4.pdf
Kugele_2-ivo3pfu89mio4.pdfGröße: 1.25 MBDownloads: 74

Datum

2022

Autor:innen

Kugele, Anandi
Uzun, Buket
Müller, Lena
Schott-Verdugo, Stephan
Gohlke, Holger
Groth, Georg
Drescher, Malte

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

DOI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Gold
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

RSC Advances. Royal Society of Chemistry. 2022, 12(12), pp. 7352-7356. eISSN 2046-2069. Available under: doi: 10.1039/d2ra00604a

Zusammenfassung

The plant ethylene receptor ETR1 is a key player in the perception of the phytohormone and subsequent downstream ethylene signal transmission, crucial for processes such as ripening, senescence and abscission. However, to date, there is sparse structural knowledge about the transmembrane sensor domain (TMD) of ETR1 that is responsible for the binding of the plant hormone and initiates the downstream signal transmission. Sequence information and ab initio modelling suggest that the TMD consists of three transmembrane helices. Here, we combined site-directed spin labelling with electron paramagnetic resonance spectroscopy and obtained distance restraints for liposome-reconstituted ETR1_TMD on the orientation and arrangement of the transmembrane helices. We used these data to scrutinize different computational structure predictions of the TMD.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690KUGELE, Anandi, Buket UZUN, Lena MÜLLER, Stephan SCHOTT-VERDUGO, Holger GOHLKE, Georg GROTH, Malte DRESCHER, 2022. Mapping the helix arrangement of the reconstituted ETR1 ethylene receptor transmembrane domain by EPR spectroscopy. In: RSC Advances. Royal Society of Chemistry. 2022, 12(12), pp. 7352-7356. eISSN 2046-2069. Available under: doi: 10.1039/d2ra00604a
BibTex
@article{Kugele2022-03-01Mappi-57007,
  year={2022},
  doi={10.1039/d2ra00604a},
  title={Mapping the helix arrangement of the reconstituted ETR1 ethylene receptor transmembrane domain by EPR spectroscopy},
  number={12},
  volume={12},
  journal={RSC Advances},
  pages={7352--7356},
  author={Kugele, Anandi and Uzun, Buket and Müller, Lena and Schott-Verdugo, Stephan and Gohlke, Holger and Groth, Georg and Drescher, Malte},
  note={This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) project no. 267205415/CRC 1208 grant to GG (TP B06) and HG (TP 03).}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/57007">
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:title>Mapping the helix arrangement of the reconstituted ETR1 ethylene receptor transmembrane domain by EPR spectroscopy</dcterms:title>
    <dc:rights>Attribution 3.0 Unported</dc:rights>
    <dc:creator>Müller, Lena</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-25T10:54:18Z</dc:date>
    <dc:contributor>Uzun, Buket</dc:contributor>
    <dc:language>eng</dc:language>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/3.0/"/>
    <dc:creator>Drescher, Malte</dc:creator>
    <dcterms:abstract xml:lang="eng">The plant ethylene receptor ETR1 is a key player in the perception of the phytohormone and subsequent downstream ethylene signal transmission, crucial for processes such as ripening, senescence and abscission. However, to date, there is sparse structural knowledge about the transmembrane sensor domain (TMD) of ETR1 that is responsible for the binding of the plant hormone and initiates the downstream signal transmission. Sequence information and ab initio modelling suggest that the TMD consists of three transmembrane helices. Here, we combined site-directed spin labelling with electron paramagnetic resonance spectroscopy and obtained distance restraints for liposome-reconstituted ETR1_TMD on the orientation and arrangement of the transmembrane helices. We used these data to scrutinize different computational structure predictions of the TMD.</dcterms:abstract>
    <dc:contributor>Groth, Georg</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/57007/1/Kugele_2-ivo3pfu89mio4.pdf"/>
    <dc:contributor>Kugele, Anandi</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/57007"/>
    <dc:creator>Schott-Verdugo, Stephan</dc:creator>
    <dc:creator>Groth, Georg</dc:creator>
    <dc:creator>Uzun, Buket</dc:creator>
    <dc:contributor>Drescher, Malte</dc:contributor>
    <dcterms:issued>2022-03-01</dcterms:issued>
    <dc:contributor>Gohlke, Holger</dc:contributor>
    <dc:contributor>Schott-Verdugo, Stephan</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/57007/1/Kugele_2-ivo3pfu89mio4.pdf"/>
    <dc:contributor>Müller, Lena</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-25T10:54:18Z</dcterms:available>
    <dc:creator>Kugele, Anandi</dc:creator>
    <dc:creator>Gohlke, Holger</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) project no. 267205415/CRC 1208 grant to GG (TP B06) and HG (TP 03).
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen