Publikation:

Controlling Protein Nanocage Assembly with Hydrostatic Pressure

Lade...
Vorschaubild

Dateien

LeVay_2-gmkvr9k2dvjx5.pdf
LeVay_2-gmkvr9k2dvjx5.pdfGröße: 407.92 KBDownloads: 128

Datum

2020

Autor:innen

Le Vay, Kristian
Carter, Ben M.
Watkins, Daniel W.
Tang, Tsing-Young Dora
Ting, Valeska P.
Rambo, Robert P.
Smith, Andrew J.
Anderson, J. L. Ross
Perriman, Adam W.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Journal of the American Chemical Society. American Chemical Society (ACS). 2020, 142(49), pp. 20640-20650. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/jacs.0c07285

Zusammenfassung

Controlling the assembly and disassembly of nanoscale protein cages for the capture and internalization of protein or non-proteinaceous components is fundamentally important to a diverse range of bionanotechnological applications. Here, we study the reversible, pressure-induced dissociation of a natural protein nanocage, E. coli bacterioferritin (Bfr), using synchrotron radiation small-angle X-ray scattering (SAXS) and circular dichroism (CD). We demonstrate that hydrostatic pressures of 450 MPa are sufficient to completely dissociate the Bfr 24-mer into protein dimers, and the reversibility and kinetics of the reassembly process can be controlled by selecting appropriate buffer conditions. We also demonstrate that the heme B prosthetic group present at the subunit dimer interface influences the stability and pressure lability of the cage, despite its location being discrete from the interdimer interface that is key to cage assembly. This indicates a major cage-stabilizing role for heme within this family of ferritins.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690LE VAY, Kristian, Ben M. CARTER, Daniel W. WATKINS, Tsing-Young Dora TANG, Valeska P. TING, Helmut CÖLFEN, Robert P. RAMBO, Andrew J. SMITH, J. L. Ross ANDERSON, Adam W. PERRIMAN, 2020. Controlling Protein Nanocage Assembly with Hydrostatic Pressure. In: Journal of the American Chemical Society. American Chemical Society (ACS). 2020, 142(49), pp. 20640-20650. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/jacs.0c07285
BibTex
@article{LeVay2020-12-09Contr-52726,
  year={2020},
  doi={10.1021/jacs.0c07285},
  title={Controlling Protein Nanocage Assembly with Hydrostatic Pressure},
  number={49},
  volume={142},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={20640--20650},
  author={Le Vay, Kristian and Carter, Ben M. and Watkins, Daniel W. and Tang, Tsing-Young Dora and Ting, Valeska P. and Cölfen, Helmut and Rambo, Robert P. and Smith, Andrew J. and Anderson, J. L. Ross and Perriman, Adam W.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52726">
    <dcterms:title>Controlling Protein Nanocage Assembly with Hydrostatic Pressure</dcterms:title>
    <dcterms:issued>2020-12-09</dcterms:issued>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Cölfen, Helmut</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Ting, Valeska P.</dc:creator>
    <dc:contributor>Ting, Valeska P.</dc:contributor>
    <dc:creator>Rambo, Robert P.</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-05T07:55:55Z</dc:date>
    <dc:contributor>Le Vay, Kristian</dc:contributor>
    <dc:creator>Le Vay, Kristian</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/52726/1/LeVay_2-gmkvr9k2dvjx5.pdf"/>
    <dc:contributor>Tang, Tsing-Young Dora</dc:contributor>
    <dc:contributor>Anderson, J. L. Ross</dc:contributor>
    <dcterms:abstract xml:lang="eng">Controlling the assembly and disassembly of nanoscale protein cages for the capture and internalization of protein or non-proteinaceous components is fundamentally important to a diverse range of bionanotechnological applications. Here, we study the reversible, pressure-induced dissociation of a natural protein nanocage, E. coli bacterioferritin (Bfr), using synchrotron radiation small-angle X-ray scattering (SAXS) and circular dichroism (CD). We demonstrate that hydrostatic pressures of 450 MPa are sufficient to completely dissociate the Bfr 24-mer into protein dimers, and the reversibility and kinetics of the reassembly process can be controlled by selecting appropriate buffer conditions. We also demonstrate that the heme B prosthetic group present at the subunit dimer interface influences the stability and pressure lability of the cage, despite its location being discrete from the interdimer interface that is key to cage assembly. This indicates a major cage-stabilizing role for heme within this family of ferritins.</dcterms:abstract>
    <dc:contributor>Carter, Ben M.</dc:contributor>
    <dc:creator>Watkins, Daniel W.</dc:creator>
    <dc:contributor>Rambo, Robert P.</dc:contributor>
    <dc:contributor>Cölfen, Helmut</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Anderson, J. L. Ross</dc:creator>
    <dc:creator>Carter, Ben M.</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-05T07:55:55Z</dcterms:available>
    <dc:creator>Smith, Andrew J.</dc:creator>
    <dc:language>eng</dc:language>
    <dc:creator>Tang, Tsing-Young Dora</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/52726/1/LeVay_2-gmkvr9k2dvjx5.pdf"/>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/52726"/>
    <dc:contributor>Watkins, Daniel W.</dc:contributor>
    <dc:contributor>Perriman, Adam W.</dc:contributor>
    <dc:contributor>Smith, Andrew J.</dc:contributor>
    <dc:creator>Perriman, Adam W.</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen