Not4-dependent translational repression is important for cellular protein homeostasis in yeast

Translation of aberrant or problematic mRNAs can cause ribosome stalling which leads to the production of truncated or defective proteins. Therefore, cells evolved cotranslational quality control mechanisms that eliminate these transcripts and target arrested nascent polypeptides for proteasomal degradation. Here we show that Not4, which is part of the multifunctional Ccr4-Not complex in yeast, associates with polysomes and contributes to the negative regulation of protein synthesis. Not4 is involved in translational repression of transcripts that cause transient ribosome stalling. The absence of Not4 affected global translational repression upon nutrient withdrawal, enhanced the expression of arrested nascent polypeptides and caused constitutive protein folding stress and aggregation. Similar defects were observed in cells with impaired mRNA decapping protein function and in cells lacking the mRNA decapping activator and translational repressor Dhh1. The results suggest a role for Not4 together with components of the decapping machinery in the regulation of protein expression on the mRNA level and emphasize the importance of translational repression for the maintenance of proteome integrity.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Ccr4–Not complex, Not4, protein homeostasis, ribosome stalling, translational repression

Zitieren

ISO 690

PREISSLER, Steffen, Julia REUTHER, Miriam KOCH, Annika SCIOR, Michael BRUDEREK, Tancred FRICKEY, Elke DEUERLING, 2015. Not4-dependent translational repression is important for cellular protein homeostasis in yeast. In: The EMBO Journal. 2015, 34(14), pp. 1905-1924. ISSN 0261-4189. eISSN 1460-2075. Available under: doi: 10.15252/embj.201490194

BibTex

@article{Preissler2015Not4d-31894,
  year={2015},
  doi={10.15252/embj.201490194},
  title={Not4-dependent translational repression is important for cellular protein homeostasis in yeast},
  number={14},
  volume={34},
  issn={0261-4189},
  journal={The EMBO Journal},
  pages={1905--1924},
  author={Preissler, Steffen and Reuther, Julia and Koch, Miriam and Scior, Annika and Bruderek, Michael and Frickey, Tancred and Deuerling, Elke}
}

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