Surface Plasmon Resonance to Measure Interactions of UbFs with Their Binding Partners

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2012
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Stingele, Julian
Roder, Uwe W.
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DOHMEN, R. Jürgen, ed., Martin SCHEFFNER, ed.. Ubiquitin family modifiers and the proteasome : reviews and protocols. New York: Humana Press, 2012, pp. 263-277. Methods in Molecular Biology. 832. ISBN 978-1-61779-473-5. Available under: doi: 10.1007/978-1-61779-474-2_19
Zusammenfassung

Ubiquitin family modifiers (UbFs) are protein–protein interaction modules acting within a variety of cellular processes. In combination with other techniques, surface plasmon resonance (SPR)-based technology has been used to characterize the interactions of UbFs with their binding partners. SPR binding assays allow the real-time detection of binding events with unlabeled analytes, yet are often hindered by the requirement for careful sample preparation and optimized assay conditions. This chapter aims to share our experience in SPR analysis of UbFs and provide helpful hints in sample preparation, experimental design, evaluation, and data interpretation.

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570 Biowissenschaften, Biologie
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ISO 690STINGELE, Julian, Uwe W. RODER, Shahri RAASI, 2012. Surface Plasmon Resonance to Measure Interactions of UbFs with Their Binding Partners. In: DOHMEN, R. Jürgen, ed., Martin SCHEFFNER, ed.. Ubiquitin family modifiers and the proteasome : reviews and protocols. New York: Humana Press, 2012, pp. 263-277. Methods in Molecular Biology. 832. ISBN 978-1-61779-473-5. Available under: doi: 10.1007/978-1-61779-474-2_19
BibTex
@incollection{Stingele2012Surfa-49290,
  year={2012},
  doi={10.1007/978-1-61779-474-2_19},
  title={Surface Plasmon Resonance to Measure Interactions of UbFs with Their Binding Partners},
  number={832},
  isbn={978-1-61779-473-5},
  publisher={Humana Press},
  address={New York},
  series={Methods in Molecular Biology},
  booktitle={Ubiquitin family modifiers and the proteasome : reviews and protocols},
  pages={263--277},
  editor={Dohmen, R. Jürgen and Scheffner, Martin},
  author={Stingele, Julian and Roder, Uwe W. and Raasi, Shahri}
}
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