Mechanism of Nanoparticle-Enhanced Turbidimetric Assays Applying Nanoparticles of Different Size and Immunoreactivity

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2002
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Eda, Shinichi
Kobold, Uwe
Kaufmann, Jörg
Puhlmann, Angela
Göltner, Christine
Wachernig, Hanno
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Langmuir. 2002, 18(20), pp. 7623-7628. ISSN 0743-7463. eISSN 1520-5827. Available under: doi: 10.1021/la025983n
Zusammenfassung

The mechanism of a novel assay technique for nanoparticle-enhanced immunoturbidimetric assays is investigated. In a mixture of latex particles of two different sizes, coated with antibodies of different affinities, the aggregation behavior is monitored, which correlates with the antigen concentration. At low antigen concentrations, only the bigger latex particles coated with the high-reactivity antibody aggregate, whereas at higher antigen concentrations, the smaller latices coated with a lower reactivity antibody follow up in the aggregation process. This is shown for an immunoassay (C-reactive protein) by theoretical considerations based on a diffusion-controlled reaction and by transmission electron microscopy, analytical ultracentrifugation, and static light scattering as complementary qualitative and quantitative analytical techniques.

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ISO 690CÖLFEN, Helmut, Antje VÖLKEL, Shinichi EDA, Uwe KOBOLD, Jörg KAUFMANN, Angela PUHLMANN, Christine GÖLTNER, Hanno WACHERNIG, 2002. Mechanism of Nanoparticle-Enhanced Turbidimetric Assays Applying Nanoparticles of Different Size and Immunoreactivity. In: Langmuir. 2002, 18(20), pp. 7623-7628. ISSN 0743-7463. eISSN 1520-5827. Available under: doi: 10.1021/la025983n
BibTex
@article{Colfen2002-10Mecha-40471,
  year={2002},
  doi={10.1021/la025983n},
  title={Mechanism of Nanoparticle-Enhanced Turbidimetric Assays Applying Nanoparticles of Different Size and Immunoreactivity},
  number={20},
  volume={18},
  issn={0743-7463},
  journal={Langmuir},
  pages={7623--7628},
  author={Cölfen, Helmut and Völkel, Antje and Eda, Shinichi and Kobold, Uwe and Kaufmann, Jörg and Puhlmann, Angela and Göltner, Christine and Wachernig, Hanno}
}
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    <dcterms:abstract xml:lang="eng">The mechanism of a novel assay technique for nanoparticle-enhanced immunoturbidimetric assays is investigated. In a mixture of latex particles of two different sizes, coated with antibodies of different affinities, the aggregation behavior is monitored, which correlates with the antigen concentration. At low antigen concentrations, only the bigger latex particles coated with the high-reactivity antibody aggregate, whereas at higher antigen concentrations, the smaller latices coated with a lower reactivity antibody follow up in the aggregation process. This is shown for an immunoassay (C-reactive protein) by theoretical considerations based on a diffusion-controlled reaction and by transmission electron microscopy, analytical ultracentrifugation, and static light scattering as complementary qualitative and quantitative analytical techniques.</dcterms:abstract>
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