Mechanism of Nanoparticle-Enhanced Turbidimetric Assays Applying Nanoparticles of Different Size and Immunoreactivity

Kurzanzeige
dc.contributor.authorCölfen, Helmut
dc.contributor.authorVölkel, Antje
dc.contributor.authorEda, Shinichi
dc.contributor.authorKobold, Uwe
dc.contributor.authorKaufmann, Jörg
dc.contributor.authorPuhlmann, Angela
dc.contributor.authorGöltner, Christine
dc.contributor.authorWachernig, Hanno
dc.date.accessioned2017-11-02T10:44:35Z
dc.date.available2017-11-02T10:44:35Z
dc.date.issued2002-10eng
dc.description.abstractThe mechanism of a novel assay technique for nanoparticle-enhanced immunoturbidimetric assays is investigated. In a mixture of latex particles of two different sizes, coated with antibodies of different affinities, the aggregation behavior is monitored, which correlates with the antigen concentration. At low antigen concentrations, only the bigger latex particles coated with the high-reactivity antibody aggregate, whereas at higher antigen concentrations, the smaller latices coated with a lower reactivity antibody follow up in the aggregation process. This is shown for an immunoassay (C-reactive protein) by theoretical considerations based on a diffusion-controlled reaction and by transmission electron microscopy, analytical ultracentrifugation, and static light scattering as complementary qualitative and quantitative analytical techniques.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1021/la025983neng
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/40471
dc.language.isoengeng
dc.subject.ddc540eng
dc.titleMechanism of Nanoparticle-Enhanced Turbidimetric Assays Applying Nanoparticles of Different Size and Immunoreactivityeng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Colfen2002-10Mecha-40471,
  year={2002},
  doi={10.1021/la025983n},
  title={Mechanism of Nanoparticle-Enhanced Turbidimetric Assays Applying Nanoparticles of Different Size and Immunoreactivity},
  number={20},
  volume={18},
  issn={0743-7463},
  journal={Langmuir},
  pages={7623--7628},
  author={Cölfen, Helmut and Völkel, Antje and Eda, Shinichi and Kobold, Uwe and Kaufmann, Jörg and Puhlmann, Angela and Göltner, Christine and Wachernig, Hanno}
}
kops.citation.iso690CÖLFEN, Helmut, Antje VÖLKEL, Shinichi EDA, Uwe KOBOLD, Jörg KAUFMANN, Angela PUHLMANN, Christine GÖLTNER, Hanno WACHERNIG, 2002. Mechanism of Nanoparticle-Enhanced Turbidimetric Assays Applying Nanoparticles of Different Size and Immunoreactivity. In: Langmuir. 2002, 18(20), pp. 7623-7628. ISSN 0743-7463. eISSN 1520-5827. Available under: doi: 10.1021/la025983ndeu
kops.citation.iso690CÖLFEN, Helmut, Antje VÖLKEL, Shinichi EDA, Uwe KOBOLD, Jörg KAUFMANN, Angela PUHLMANN, Christine GÖLTNER, Hanno WACHERNIG, 2002. Mechanism of Nanoparticle-Enhanced Turbidimetric Assays Applying Nanoparticles of Different Size and Immunoreactivity. In: Langmuir. 2002, 18(20), pp. 7623-7628. ISSN 0743-7463. eISSN 1520-5827. Available under: doi: 10.1021/la025983neng
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