The presence of amino acids affects inorganic N uptake in non-mycorrhizal seedlings of European beech (Fagus sylvatica)

Lade...
Vorschaubild
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2010
Autor:innen
Stoelken, Gunda
Ehlting, Barbara
Rennenberg, Heinz
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Tree Physiology. 2010, 30(9), pp. 1118-1128. ISSN 0829-318X. eISSN 1758-4469. Available under: doi: 10.1093/treephys/tpq050
Zusammenfassung

To investigate the impact of organic N compounds for inorganic nitrogen uptake in the rhizosphere, we fed ammonium nitrate with or without amino acids (i.e., glutamine or arginine) to the roots of non-mycorrhizal beech (Fagus sylvatica L.) seedlings under controlled conditions at different levels of N availability. Uptake of individual N sources was determined from ¹⁵N (inorganic N) and ¹⁵N ¹³C (organic N) accumulation in the roots. In addition, gene fragments encoding proteins involved in N uptake and metabolism were cloned from beech for gene expression analyses by quantitative real-time PCR in the roots. Generally, ammonium was preferred over nitrate as N source. Organic N sources were taken up by beech roots as intact molecules. Uptake of organic N was significantly higher than inorganic N uptake, thus contributing significantly to N nutrition of beech. Depending on the level of N availability, inorganic N uptake was negatively affected by the presence of organic N sources. This result indicates an overestimation of the contribution of inorganic N uptake to N nutrition of beech in previous studies. Apparently, association with mycorrhizal fungi is not essential for organic N uptake by beech roots. Gene expression analyses showed that transcriptional regulation of the amino acid transporters FsCAT3, FsCAT5, FsAAT and FsAAP and the ammonium transporter FsAMT1.2 in the roots is involved in N nutrition of beech.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690STOELKEN, Gunda, Judy SIMON, Barbara EHLTING, Heinz RENNENBERG, 2010. The presence of amino acids affects inorganic N uptake in non-mycorrhizal seedlings of European beech (Fagus sylvatica). In: Tree Physiology. 2010, 30(9), pp. 1118-1128. ISSN 0829-318X. eISSN 1758-4469. Available under: doi: 10.1093/treephys/tpq050
BibTex
@article{Stoelken2010-09prese-43238,
  year={2010},
  doi={10.1093/treephys/tpq050},
  title={The presence of amino acids affects inorganic N uptake in non-mycorrhizal seedlings of European beech (Fagus sylvatica)},
  number={9},
  volume={30},
  issn={0829-318X},
  journal={Tree Physiology},
  pages={1118--1128},
  author={Stoelken, Gunda and Simon, Judy and Ehlting, Barbara and Rennenberg, Heinz}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/43238">
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-09-12T06:56:46Z</dcterms:available>
    <dc:contributor>Rennenberg, Heinz</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-09-12T06:56:46Z</dc:date>
    <dcterms:title>The presence of amino acids affects inorganic N uptake in non-mycorrhizal seedlings of European beech (Fagus sylvatica)</dcterms:title>
    <dc:contributor>Stoelken, Gunda</dc:contributor>
    <dc:creator>Simon, Judy</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Simon, Judy</dc:contributor>
    <dc:creator>Ehlting, Barbara</dc:creator>
    <dcterms:abstract xml:lang="eng">To investigate the impact of organic N compounds for inorganic nitrogen uptake in the rhizosphere, we fed ammonium nitrate with or without amino acids (i.e., glutamine or arginine) to the roots of non-mycorrhizal beech (Fagus sylvatica L.) seedlings under controlled conditions at different levels of N availability. Uptake of individual N sources was determined from ¹⁵N (inorganic N) and ¹⁵N ¹³C (organic N) accumulation in the roots. In addition, gene fragments encoding proteins involved in N uptake and metabolism were cloned from beech for gene expression analyses by quantitative real-time PCR in the roots. Generally, ammonium was preferred over nitrate as N source. Organic N sources were taken up by beech roots as intact molecules. Uptake of organic N was significantly higher than inorganic N uptake, thus contributing significantly to N nutrition of beech. Depending on the level of N availability, inorganic N uptake was negatively affected by the presence of organic N sources. This result indicates an overestimation of the contribution of inorganic N uptake to N nutrition of beech in previous studies. Apparently, association with mycorrhizal fungi is not essential for organic N uptake by beech roots. Gene expression analyses showed that transcriptional regulation of the amino acid transporters FsCAT3, FsCAT5, FsAAT and FsAAP and the ammonium transporter FsAMT1.2 in the roots is involved in N nutrition of beech.</dcterms:abstract>
    <dc:contributor>Ehlting, Barbara</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Rennenberg, Heinz</dc:creator>
    <dcterms:issued>2010-09</dcterms:issued>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/43238"/>
    <dc:creator>Stoelken, Gunda</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Ja
Diese Publikation teilen