Enhanced accuracy of single-molecule diffusion measurements with a photocleavable energy-transfer dyad

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Angewandte Chemie International Edition. 2013, 52(47), pp. 12435-12438. ISSN 1433-7851. eISSN 1521-3773. Available under: doi: 10.1002/anie.201303658
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A photocleavable energy-transfer dyad was synthesized, characterized, and applied to single-molecule fluorescence microscopy. After photocleavage, a combination of independent two-color single-molecule tracking and analysis of single-molecule energy-transfer efficiencies allows the determination of the temporal evolution of the relative distances between both fragments from the nm to the μm scale. This gives access to a broad range of diffusion coefficients.

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ISO 690DILL, Maren, Moritz C. BAIER, Stefan MECKING, Dominik WÖLL, 2013. Enhanced accuracy of single-molecule diffusion measurements with a photocleavable energy-transfer dyad. In: Angewandte Chemie International Edition. 2013, 52(47), pp. 12435-12438. ISSN 1433-7851. eISSN 1521-3773. Available under: doi: 10.1002/anie.201303658
BibTex
@article{Dill2013-11-18Enhan-24499,
  year={2013},
  doi={10.1002/anie.201303658},
  title={Enhanced accuracy of single-molecule diffusion measurements with a photocleavable energy-transfer dyad},
  number={47},
  volume={52},
  issn={1433-7851},
  journal={Angewandte Chemie International Edition},
  pages={12435--12438},
  author={Dill, Maren and Baier, Moritz C. and Mecking, Stefan and Wöll, Dominik}
}
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