Relaxation-based distance measurements between a nitroxide and a lanthanide spin label

No Thumbnail Available
Files
There are no files associated with this item.
Date
2008
Authors
Jäger, Heidrun
Koch, Achim
Maus, Verona
Spiess, Hans Wolfgang
Editors
Contact
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
ArXiv-ID
International patent number
Link to the license
oops
EU project number
Project
Open Access publication
Collections
Restricted until
Title in another language
Research Projects
Organizational Units
Journal Issue
Publication type
Journal article
Publication status
Published
Published in
Journal of Magnetic Resonance ; 194 (2008), 2. - pp. 254-263. - Elsevier. - ISSN 1090-7807. - eISSN 1096-0856
Abstract
Distance measurements by electron paramagnetic resonance techniques between labels attached to biomacromolecules provide structural information on systems that cannot be crystallized or are too large to be characterized by NMR methods. However, existing techniques are limited in their distance range and sensitivity. It is anticipated by theoretical considerations that these limits could be extended by measuring the enhancement of longitudinal relaxation of a nitroxide label due to a lanthanide complex label at cryogenic temperatures. The relaxivity of the dysprosium complex with the macrocyclic ligand DOTA can be determined without direct measurements of longitudinal relaxation rates of the lanthanide and without recourse to model compounds with well defined distance by analyzing the dependence of relaxation enhancement on either temperature or concentration in homogeneous glassy frozen solutions. Relaxivities determined by the two calibration techniques are in satisfying agreement with each other. Error sources for both techniques are examined. A distance of about 2.7 nm is measured in a model compound of the type nitroxide–spacer–lanthanide complex and is found in good agreement with the distance in a modeled structure. Theoretical considerations suggest that an increase of the upper distance limit requires measurements at lower fields and temperatures.
Summary in another language
Subject (DDC)
540 Chemistry
Keywords
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690JÄGER, Heidrun, Achim KOCH, Verona MAUS, Hans Wolfgang SPIESS, Gunnar JESCHKE, 2008. Relaxation-based distance measurements between a nitroxide and a lanthanide spin label. In: Journal of Magnetic Resonance. Elsevier. 194(2), pp. 254-263. ISSN 1090-7807. eISSN 1096-0856. Available under: doi: 10.1016/j.jmr.2008.07.012
BibTex
@article{Jager2008-10Relax-58711,
  year={2008},
  doi={10.1016/j.jmr.2008.07.012},
  title={Relaxation-based distance measurements between a nitroxide and a lanthanide spin label},
  number={2},
  volume={194},
  issn={1090-7807},
  journal={Journal of Magnetic Resonance},
  pages={254--263},
  author={Jäger, Heidrun and Koch, Achim and Maus, Verona and Spiess, Hans Wolfgang and Jeschke, Gunnar}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/58711">
    <dc:contributor>Jeschke, Gunnar</dc:contributor>
    <dcterms:abstract xml:lang="eng">Distance measurements by electron paramagnetic resonance techniques between labels attached to biomacromolecules provide structural information on systems that cannot be crystallized or are too large to be characterized by NMR methods. However, existing techniques are limited in their distance range and sensitivity. It is anticipated by theoretical considerations that these limits could be extended by measuring the enhancement of longitudinal relaxation of a nitroxide label due to a lanthanide complex label at cryogenic temperatures. The relaxivity of the dysprosium complex with the macrocyclic ligand DOTA can be determined without direct measurements of longitudinal relaxation rates of the lanthanide and without recourse to model compounds with well defined distance by analyzing the dependence of relaxation enhancement on either temperature or concentration in homogeneous glassy frozen solutions. Relaxivities determined by the two calibration techniques are in satisfying agreement with each other. Error sources for both techniques are examined. A distance of about 2.7 nm is measured in a model compound of the type nitroxide–spacer–lanthanide complex and is found in good agreement with the distance in a modeled structure. Theoretical considerations suggest that an increase of the upper distance limit requires measurements at lower fields and temperatures.</dcterms:abstract>
    <dc:contributor>Spiess, Hans Wolfgang</dc:contributor>
    <dc:creator>Spiess, Hans Wolfgang</dc:creator>
    <dc:creator>Maus, Verona</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-09-30T07:44:28Z</dcterms:available>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Koch, Achim</dc:creator>
    <dcterms:title>Relaxation-based distance measurements between a nitroxide and a lanthanide spin label</dcterms:title>
    <dc:contributor>Koch, Achim</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/58711"/>
    <dc:contributor>Maus, Verona</dc:contributor>
    <dcterms:issued>2008-10</dcterms:issued>
    <dc:language>eng</dc:language>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-09-30T07:44:28Z</dc:date>
    <dc:contributor>Jäger, Heidrun</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Jäger, Heidrun</dc:creator>
    <dc:creator>Jeschke, Gunnar</dc:creator>
  </rdf:Description>
</rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Contact
URL of original publication
Test date of URL
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes
Refereed
Yes