@article{Isono2004-06-25Requi-42151,
  year={2004},
  doi={10.1074/jbc.M314231200},
  title={Rpn7 Is Required for the Structural Integrity of the 26 S Proteasome of Saccharomyces cerevisiae},
  number={26},
  volume={279},
  issn={0021-9258},
  journal={The Journal of Biological Chemistry : JBC},
  pages={27168--27176},
  author={Isono, Erika and Saeki, Yasushi and Yokosawa, Hideyoshi and Toh-e, Akio}
}

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    <dcterms:abstract xml:lang="eng">Rpn7 is one of the lid subunits of the 26 S proteasome regulatory particle. The RPN7 gene is known to be essential, but its function remains to be elucidated. To explore the function of Rpn7, we isolated and characterized temperature-sensitive rpn7 mutants. All of the rpn7 mutants obtained accumulated poly-ubiquitinated proteins when grown at the restrictive temperature. The N-end rule substrate (Ub-Arg-beta-galactosidase), the UFD pathway substrate (Ub-Pro-beta-galactosidase), and cell cycle regulators (Pds1 and Clb2) were found to be stabilized in experiments using one of the rpn7 mutants termed rpn7-3 at the restrictive temperature, indicating its defect in the ubiquitin-proteasome pathway. Subsequent analysis of the structure of the 26 S proteasome in rpn7-3 cells suggested that the defect was in the assembly of the 26 S holoenzyme. The most striking characteristic of the proteasome of the rpn7-3 mutant was that a lid subcomplex affinity-purified from the rpn7-3 cells grown at the restrictive temperature contained only 5 of the 8 lid components, a phenomenon that has not been reported in the previously isolated lid mutants. From these results, we concluded that Rpn7 is required for the integrity of the 26 S complex by establishing a correct lid structure.</dcterms:abstract>
    <dcterms:title>Rpn7 Is Required for the Structural Integrity of the 26 S Proteasome of Saccharomyces cerevisiae</dcterms:title>
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    <dc:creator>Saeki, Yasushi</dc:creator>
    <dc:creator>Toh-e, Akio</dc:creator>
    <dc:language>eng</dc:language>
    <dc:contributor>Yokosawa, Hideyoshi</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-04-24T12:46:59Z</dc:date>
    <dc:contributor>Isono, Erika</dc:contributor>
    <dc:contributor>Toh-e, Akio</dc:contributor>
    <dcterms:issued>2004-06-25</dcterms:issued>
    <dc:creator>Yokosawa, Hideyoshi</dc:creator>
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    <dc:creator>Isono, Erika</dc:creator>
    <dc:contributor>Saeki, Yasushi</dc:contributor>
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