Small increases in corticosterone before the breeding season increase parental investment but not fitness in a wild passerine bird

Abstract

Correlative evidence from field studies has suggested that baseline concentrations of corticosterone, the main avian glucocorticoid hormone, affect reproductive strategies in vertebrate species. Such a role is conceivable in light of corticosterone's function as a metabolic hormone in regulating glucose and fat metabolism. From such correlational studies, however, the question has remained open whether glucocorticoid concentrations change in advance of reproductive activities or whether corticosterone concentrations vary passively as a consequence of the individual's reproductive investment and workload. To test such causal relationships, we manipulated corticosterone concentrations prior to the breeding season in adult great tits (Parus major) and quantified reproductive investment and success. Two weeks before egg-laying, we administered subcutaneous silastic implants filled with corticosterone that elevated circulating levels within the baseline range for approximately 30 days to adult males and females. Corticosterone manipulation did not affect lay date or yearly offspring production. However, reproductive behaviors were affected by corticosterone treatment: males fed their mates more often during incubation, and females increased incubation of eggs and brooding of nestlings compared to control individuals. Other behaviors during the nestling stage, when the implants were no longer effective, did not differ between the two treatment groups. Our findings do not support the view that baseline corticosterone concentrations, at least at the time of year when we administered implants, change reproductive strategies per se. The current data suggest that baseline corticosterone levels represent internal signals that causally mediate reproductive effort in individuals of a wild bird species. By increasing reproductive investment, baseline corticosterone concentrations may have functions during the breeding season that diverge from the suppressive effects of stress-induced concentrations.