KOPS - Das Institutionelle Repositorium der Universität Konstanz

Microarray-based multicycle-enrichment of genomic subsets for targeted next-generation sequencing

Microarray-based multicycle-enrichment of genomic subsets for targeted next-generation sequencing

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:8485e3969c764ccaf36664b75d45ad61

SUMMERER, Daniel, Haiguo WU, Bettina HAASE, Yang CHENG, Nadine SCHRACKE, Cord F. STÄHLER, Mark S. CHEE, Peer F. STÄHLER, Markus BEIER, 2009. Microarray-based multicycle-enrichment of genomic subsets for targeted next-generation sequencing. In: Genome Research. 19(9), pp. 1616-1621. ISSN 1088-9051. eISSN 1549-5469. Available under: doi: 10.1101/gr.091942.109

@article{Summerer2009-09Micro-9988, title={Microarray-based multicycle-enrichment of genomic subsets for targeted next-generation sequencing}, year={2009}, doi={10.1101/gr.091942.109}, number={9}, volume={19}, issn={1088-9051}, journal={Genome Research}, pages={1616--1621}, author={Summerer, Daniel and Wu, Haiguo and Haase, Bettina and Cheng, Yang and Schracke, Nadine and Stähler, Cord F. and Chee, Mark S. and Stähler, Peer F. and Beier, Markus} }

Schracke, Nadine Stähler, Cord F. 2011-03-24T18:15:48Z Summerer, Daniel Wu, Haiguo Stähler, Cord F. Schracke, Nadine Beier, Markus Haase, Bettina The lack of efficient high-throughput methods for enrichment of specific sequences from genomic DNA represents a key bottleneck in exploiting the enormous potential of next-generation sequencers. Such methods would allow for a systematic and targeted analysis of relevant genomic regions. Recent studies reported sequence enrichment using a hybridization step to specific DNA capture probes as a possible solution to the problem. However, so far no method has provided sufficient depths of coverage for reliable base calling over the entire target regions. We report a strategy to multiply the enrichment performance and consequently improve depth and breadth of coverage for desired target sequences by applying two iterative cycles of hybridization with microfluidic Geniom biochips. Using this strategy, we enriched and then sequenced the cancer-related genes BRCA1 and TP53 and a set of 1000 individual dbSNP regions of 500 bp using Illumina technology. We achieved overall enrichment factors of up to 1062-fold and average coverage depths of 470-fold. Combined with high coverage uniformity, this resulted in nearly complete consensus coverages with >86% of target region covered at 20-fold or higher. Analysis of SNP calling accuracies after enrichment revealed excellent concordance, with the reference sequence closely mirroring the previously reported performance of Illumina sequencing conducted without sequence enrichment. application/pdf eng First publ. in: Genome research 19 (2009), 9, pp. 1616-1621 Chee, Mark S. deposit-license Microarray-based multicycle-enrichment of genomic subsets for targeted next-generation sequencing Cheng, Yang Haase, Bettina Cheng, Yang Summerer, Daniel Wu, Haiguo Chee, Mark S. 2011-03-24T18:15:48Z 2009-09 Stähler, Peer F. Stähler, Peer F. Beier, Markus

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Genome_Res.pdf 310

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto