Aptazyme-Mediated Regulation of 16S Ribosomal RNA

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WIELAND, Markus, Barbara BERSCHNEIDER, Matthias D. ERLACHER, Jörg Steffen HARTIG, 2010. Aptazyme-Mediated Regulation of 16S Ribosomal RNA. In: Chemistry & Biology. 17(3), pp. 236-242. ISSN 1074-5521. eISSN 1879-1301. Available under: doi: 10.1016/j.chembiol.2010.02.012

@article{Wieland2010Aptaz-9945, title={Aptazyme-Mediated Regulation of 16S Ribosomal RNA}, year={2010}, doi={10.1016/j.chembiol.2010.02.012}, number={3}, volume={17}, issn={1074-5521}, journal={Chemistry & Biology}, pages={236--242}, author={Wieland, Markus and Berschneider, Barbara and Erlacher, Matthias D. and Hartig, Jörg Steffen} }

Erlacher, Matthias D. 2011-03-24T18:15:30Z Hartig, Jörg Steffen eng Wieland, Markus 2011-03-24T18:15:30Z Berschneider, Barbara Hartig, Jörg Steffen 2010 Wieland, Markus Developing artificial genetic switches in order to control gene expression via an external stimulus is an important aim in chemical and synthetic biology. Here, we expand the application range of RNA switches to the regulation of 16S rRNA function in Escherichia coli. For this purpose, we incorporated hammerhead ribozymes at several positions into orthogonalized 16S rRNA. We observed that ribosomal function is remarkably tolerant toward the incorporation of large additional RNA fragments at certain sites of the 16S rRNA. However, ribozyme-mediated cleavage results in severe reduction of 16S rRNA stability. We carried out an in vivo screen for the identification of sequences acting as ligand-responsive RNA switches, enabling thiamine-dependent switching of 16S rRNA function. In addition to expanding the regulatory toolbox, the presented artificial riboswitches should prove valuable to study aspects of rRNA folding and stability in bacteria. First publ. in: Chemistry & Biology 17 (2010), 3, pp. 236-242 application/pdf Aptazyme-Mediated Regulation of 16S Ribosomal RNA deposit-license Berschneider, Barbara Erlacher, Matthias D.

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