KOPS - Das Institutionelle Repositorium der Universität Konstanz

Lung alveolar proteomics of bronchoalveolar lavage from a pulmonary alveolar proteinosis patient using high-resolution FTICR mass spectrometry

Lung alveolar proteomics of bronchoalveolar lavage from a pulmonary alveolar proteinosis patient using high-resolution FTICR mass spectrometry

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:ade1f8565227b7180a5adf293252e856

BAI, Yu, Dmitry GALETSKIY, Eugen DAMOC, Jan RIPPER, Markus WOISCHNIK, Matthias GRIESE, Zhiqiang LIU, Shuying LIU, Michael PRZYBYLSKI, 2007. Lung alveolar proteomics of bronchoalveolar lavage from a pulmonary alveolar proteinosis patient using high-resolution FTICR mass spectrometry. In: Analytical and bioanalytical chemistry. 389(4), pp. 1075-1085. ISSN 1618-2642

@article{Bai2007alveo-9942, title={Lung alveolar proteomics of bronchoalveolar lavage from a pulmonary alveolar proteinosis patient using high-resolution FTICR mass spectrometry}, year={2007}, number={4}, volume={389}, issn={1618-2642}, journal={Analytical and bioanalytical chemistry}, pages={1075--1085}, author={Bai, Yu and Galetskiy, Dmitry and Damoc, Eugen and Ripper, Jan and Woischnik, Markus and Griese, Matthias and Liu, Zhiqiang and Liu, Shuying and Przybylski, Michael} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/9942"> <dc:creator>Damoc, Eugen</dc:creator> <dc:contributor>Bai, Yu</dc:contributor> <dcterms:issued>2007</dcterms:issued> <dc:format>application/pdf</dc:format> <dc:creator>Griese, Matthias</dc:creator> <dc:contributor>Damoc, Eugen</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:15:28Z</dcterms:available> <dcterms:title>Lung alveolar proteomics of bronchoalveolar lavage from a pulmonary alveolar proteinosis patient using high-resolution FTICR mass spectrometry</dcterms:title> <dc:contributor>Galetskiy, Dmitry</dc:contributor> <dc:contributor>Przybylski, Michael</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:creator>Galetskiy, Dmitry</dc:creator> <dc:contributor>Ripper, Jan</dc:contributor> <dc:contributor>Liu, Zhiqiang</dc:contributor> <dc:creator>Ripper, Jan</dc:creator> <dcterms:abstract xml:lang="eng">High-resolution Fourier transform ion cyclotron resonance (FTICR) mass spectrometry was developed and applied to the proteome analysis of bronchoalveolar lavage fluid (BALF) from a patient with pulmonary alveolar proteinosis. With use of 1-D and 2-D gel electrophoresis, surfactant protein A (SP-A) and other surfactant-related lung alveolar proteins were efficiently separated and identified by matrix-assisted laser desorption/ionization FTICR mass spectrometry . Low molecular mass BALF proteins were separated using a gradient 2-D gel. An efficient extraction/precipitation system was developed and used for the enrichment of surfactant proteins. The result of the BALF proteome analysis show the presence of several isoforms of SP-A, in which an N-non-glycosylierte form and several proline hydroxylations were identified. Furthermore, a number of protein spots were found to contain a mixture of proteins unresolved by 2-D gel electrophoresis, illustrating the feasibility of high-resolution mass spectrometry to provide identifications of proteins that remain unseparated in 2-D gels even upon extended pH gradients.</dcterms:abstract> <dc:language>eng</dc:language> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/9942"/> <dc:creator>Bai, Yu</dc:creator> <dc:creator>Woischnik, Markus</dc:creator> <dc:creator>Liu, Zhiqiang</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Woischnik, Markus</dc:contributor> <dc:creator>Przybylski, Michael</dc:creator> <dc:contributor>Griese, Matthias</dc:contributor> <dc:contributor>Liu, Shuying</dc:contributor> <dc:creator>Liu, Shuying</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:15:28Z</dc:date> <dcterms:bibliographicCitation>First publ. in: Analytical and bioanalytical chemistry 389 (2007), 4, pp. 1075-1085</dcterms:bibliographicCitation> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

ABC_2007_Bai.pdf 424

Das Dokument erscheint in:

deposit-license Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: deposit-license

KOPS Suche


Stöbern

Mein Benutzerkonto