Chemical Biology of DNA Polymerases : From Selectivity to New Functions

Cite This

Files in this item

Checksum: MD5:160bb3e46a3f06232dbc2608d43757e1

MARX, Andreas, Daniel SUMMERER, Katharina B. M. SAUTER, Christian GLOECKNER, Nicolas Zackes RUDINGER, 2007. Chemical Biology of DNA Polymerases : From Selectivity to New Functions. In: Nucleic acids symposium series. 51(1), pp. 81-82

@article{Marx2007Chemi-9894, title={Chemical Biology of DNA Polymerases : From Selectivity to New Functions}, year={2007}, number={1}, volume={51}, journal={Nucleic acids symposium series}, pages={81--82}, author={Marx, Andreas and Summerer, Daniel and Sauter, Katharina B. M. and Gloeckner, Christian and Rudinger, Nicolas Zackes} }

Chemical Biology of DNA Polymerases : From Selectivity to New Functions Summerer, Daniel Attribution-NonCommercial-NoDerivs 2.0 Generic eng Gloeckner, Christian Sauter, Katharina B. M. Rudinger, Nicolas Zackes Gloeckner, Christian 2007 Marx, Andreas DNA polymerases are involved in all DNA synthesis occurring in nature. Furthermore, DNA polymerases are the workhorses in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing and nucleic acids based diagnostics.<br />In order to identify DNA polymerase mutants with altered properties, we set up an efficient high through put setup to rapidly screen libraries of DNA polymerase mutants in automated parallel fashion and identified entities with significantly increased selectivity. Furthermore, our results indicate a rational to generally increase DNA polymerase selectivity as we demonstrate for several enzymes from different DNA polymerase families.<br />Additionally, we show that the generation of a new DNA polymerase function is achievable through iterative screening of small libraries of DNA polymerase derived by randomization of the respective genes. We demonstrate that the identified mutants find immediate applications and provide the basis for the development of new means for diagnostic technologies. Summerer, Daniel First publ. in: Nucleic acids symposium series ; 51 (2007), 1. - S. 81-82 Marx, Andreas 2011-03-24T18:15:09Z Rudinger, Nicolas Zackes Sauter, Katharina B. M. application/pdf

Downloads since Oct 1, 2014 (Information about access statistics)

Marx_Nucl_Acids_Symp_Ser_2007.pdf 470

This item appears in the following Collection(s)

Attribution-NonCommercial-NoDerivs 2.0 Generic Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.0 Generic

Search KOPS


My Account