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Mechanism of human alpha-1,3-fucosyltransferase V : glycosidic cleavage occurs prior to nucleophilic attack

Mechanism of human alpha-1,3-fucosyltransferase V : glycosidic cleavage occurs prior to nucleophilic attack

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MURRAY, Brion W., Valentin WITTMANN, Michael D. BURKART, Shang-Cheng HUNG, Chi-Huey WONG, 1997. Mechanism of human alpha-1,3-fucosyltransferase V : glycosidic cleavage occurs prior to nucleophilic attack. In: Biochemistry. 36(4), pp. 823-831. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/bi962284z

@article{Murray1997Mecha-9726, title={Mechanism of human alpha-1,3-fucosyltransferase V : glycosidic cleavage occurs prior to nucleophilic attack}, year={1997}, doi={10.1021/bi962284z}, number={4}, volume={36}, issn={0006-2960}, journal={Biochemistry}, pages={823--831}, author={Murray, Brion W. and Wittmann, Valentin and Burkart, Michael D. and Hung, Shang-Cheng and Wong, Chi-Huey} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/9726"> <dc:contributor>Wittmann, Valentin</dc:contributor> <dc:language>eng</dc:language> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:13:58Z</dc:date> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9726/1/Mechanism_of_human.pdf"/> <dcterms:abstract xml:lang="eng">α-1,3-Fucosyltransferase V (FucT V) catalyzes the transfer of l-fucose from the donor sugar guanosine 5'-diphospho-β-l-fucose (GDP-Fuc) to an acceptor sugar. A secondary isotope effect on the fucosyltransfer reaction with guanosine 5'-diphospho-[1-²H]-β-l-fucose (GDP-[1-²H]-Fuc) as the substrate was observed and determined to be DV = 1.32 + 0.13 and DV/K = 1.27 + 0.07. Competitive inhibition of FucT V by guanosine 5'-diphospho-2-deoxy-2-fluoro-β-l-fucose (GDP-2F-Fuc) was observed with an inhibition constant of 4.2 íM which represents the most potent inhibitor of this enzyme to date. Incubation of GDP-2F-Fuc with FucT V and an acceptor molecule prior to the addition of GDP-Fuc had no effect on the potency of inhibition, indicating that GDP-2F-Fuc is neither an inactivator nor a slow substrate. Both the observed secondary isotope effect and the inhibition by GDP-2F-Fuc are consistent with a charged, sp²-hybridized, transition-state structure. A convenient and efficient synthesis of GDP-[1-²H]-Fuc and GDP-2F-Fuc and a nonradioactive, fluorescence assay for fucosyltransferase activity have been developed.</dcterms:abstract> <dc:creator>Murray, Brion W.</dc:creator> <dc:format>application/pdf</dc:format> <dc:contributor>Hung, Shang-Cheng</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:creator>Hung, Shang-Cheng</dc:creator> <dc:rights>deposit-license</dc:rights> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:title>Mechanism of human alpha-1,3-fucosyltransferase V : glycosidic cleavage occurs prior to nucleophilic attack</dcterms:title> <dcterms:issued>1997</dcterms:issued> <dc:contributor>Wong, Chi-Huey</dc:contributor> <dc:creator>Wittmann, Valentin</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:bibliographicCitation>First publ. in: Biochemistry ; 36 (1997). - S. 823-831</dcterms:bibliographicCitation> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/9726"/> <dc:contributor>Burkart, Michael D.</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9726/1/Mechanism_of_human.pdf"/> <dc:creator>Wong, Chi-Huey</dc:creator> <dc:contributor>Murray, Brion W.</dc:contributor> <dc:creator>Burkart, Michael D.</dc:creator> </rdf:Description> </rdf:RDF>

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