Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-43333 |
Author: | Ladenburger, Eva-Maria; Korn, Iris; Kasielke, Nicole; Wassmer, Thomas; Plattner, Helmut |
Year of publication: | 2006 |
Published in: | Journal of Cell Science ; 119 (2006), 17. - pp. 3705-3717. - ISSN 0021-9533. - eISSN 1477-9137 |
Pubmed ID: | 16912081 |
DOI (citable link): | https://dx.doi.org/10.1242/jcs.03075 |
Summary: |
In the ciliate Paramecium, a variety of well characterized processes are regulated by Ca2+, e.g. exocytosis, endocytosis and ciliary beat. Therefore, among protozoa, Paramecium is considered a model organism for Ca2+ signaling, although the molecular identity of the channels responsible for the Ca2+ signals remains largely unknown. We have cloned for the first time in a protozoan the full sequence of the gene encoding a putative inositol (1,4,5)-trisphosphate (Ins(1,4,5)P3) receptor from Paramecium tetraurelia cells showing molecular characteristics of higher eukaryotic cells. The homologously expressed Ins(1,4,5)P3-binding domain binds [3H]Ins(1,4,5)P3, whereas antibodies unexpectedly localize this protein to the osmoregulatorysystem. The level of Ins(1,4,5)P3-receptor expression was reduced, as shown on a transcriptional level and by immuno-staining, by decreasing the concentration of extracellular Ca2+ (Paramecium cells rapidly adjust their Ca2+ level to that in the outside medium). Fluorochromes reveal spontaneous fluctuations in cytosolic Ca2+ levels along the osmoregulatory system and these signals change upon activation of caged Ins(1,4,5)P3. Considering the ongoing expulsion of substantial amounts of Ca2+ by the osmoregulatory system, we propose here that Ins(1,4,5)P3 receptors serve a new function, i.e. a latent, graded reflux of Ca2+ to fine-tune [Ca2+] homeostasis.
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Subject (DDC): | 540 Chemistry |
Keywords: | Ca2+, Calcium, Inositol 1,4,5-trisphosphate, IP3, Osmoregulation, Paramecium |
Link to License: | Attribution-NonCommercial-NoDerivs 2.0 Generic |
Bibliography of Konstanz: | Yes |
LADENBURGER, Eva-Maria, Iris KORN, Nicole KASIELKE, Thomas WASSMER, Helmut PLATTNER, 2006. An Ins(1,4,5)P3 receptor in Paramecium is associatedwith the osmoregulatory system. In: Journal of Cell Science. 119(17), pp. 3705-3717. ISSN 0021-9533. eISSN 1477-9137. Available under: doi: 10.1242/jcs.03075
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P3_receptor_in_Paramecium_is_associatedwith_the_osmoregulatory_system.pdf | 615 |