Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors
| Type of Publication: | Journal article |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-125820 |
| Author: | Lei, Ming; Zumbusch, Andreas |
| Year of publication: | 2010 |
| Published in: | Optics Letters ; 35 (2010), 23. - pp. 4057-4059. - ISSN 0146-9592. - eISSN 1539-4794 |
| Pubmed ID: | 21124611 |
| DOI (citable link): | https://dx.doi.org/10.1364/OL.35.004057 |
| Summary: |
A scheme based on a W-shaped axicon mirror device for total-internal-reflection fluorescence microscopy (TIRFM)is presented. This approach combines the advantages of higher efficiency compared with traditional TIRFM, adjustable illumination area, and simple switching between wide-field and TIRF imaging modes. TIRF images obtained with this approach are free of shadow artifacts and of interference fringes. Example micrographs of fluorescently labeled polystyrene beads, of Convallaria majalis tissue, and of Propidium-iodide-labeled Chinese hamster ovary cells are shown, and the capabilities of the scheme are discussed.
|
| Subject (DDC): | 540 Chemistry |
| Link to License: | Terms of use |
| Bibliography of Konstanz: | Yes |
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LEI, Ming, Andreas ZUMBUSCH, 2010. Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors. In: Optics Letters. 35(23), pp. 4057-4059. ISSN 0146-9592. eISSN 1539-4794. Available under: doi: 10.1364/OL.35.004057
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