Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-107686 |
Author: | Semmler, Angelika; Weber, Reinhold; Przybylski, Michael; Wittmann, Valentin |
Year of publication: | 2010 |
Published in: | Journal of the American Society for Mass Spectrometry ; 21 (2010), 2. - pp. 215-219. - ISSN 1044-0305. - eISSN 1879-1123 |
Pubmed ID: | 19914846 |
DOI (citable link): | https://dx.doi.org/10.1016/j.jasms.2009.10.004 |
Summary: |
An efficient approach in combinatorial chemistry is the synthesis of one-bead-one-compound peptide libraries. In contrast to synthesis and functional screening, which is performed in a largely automated manner, structure determination has been frequently laborious and time-consuming. Here we report an approach for de novo sequencing of peptides on single beads by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance (MALDI-FTICR) tandem mass spectrometry, using a resin with a photolinker for solid-phase peptide synthesis. Upon sorting out single beads, an efficient sample preparation on the MALDI target was developed that enables fragmentation upon irradiation of the bead-matrix mixture with the ultraviolet (UV)-MALDI laser, with enhanced yield of sequence-specific fragment ions at increased laser energy. This approach is illustrated by sequence determinations of two peptides from a library with sequences varying in a single amino acid; the feasibility with tandem-MS procedures and fragment ion assignment was ascertained by sustained off-resonance irradiation/collision induced dissociation (SORI/CID) and infrared multiphoton dissociation (IRMPD) fragmentation.
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Subject (DDC): | 540 Chemistry |
Link to License: | In Copyright |
Bibliography of Konstanz: | Yes |
SEMMLER, Angelika, Reinhold WEBER, Michael PRZYBYLSKI, Valentin WITTMANN, 2010. De novo sequencing of peptides on single resin beads by MALDI-FTICR tandem mass spectrometry. In: Journal of the American Society for Mass Spectrometry. 21(2), pp. 215-219. ISSN 1044-0305. eISSN 1879-1123. Available under: doi: 10.1016/j.jasms.2009.10.004
@article{Semmler2010seque-9644, title={De novo sequencing of peptides on single resin beads by MALDI-FTICR tandem mass spectrometry}, year={2010}, doi={10.1016/j.jasms.2009.10.004}, number={2}, volume={21}, issn={1044-0305}, journal={Journal of the American Society for Mass Spectrometry}, pages={215--219}, author={Semmler, Angelika and Weber, Reinhold and Przybylski, Michael and Wittmann, Valentin} }
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Przybylski_Michael2009_k.pdf | 336 |