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Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry

Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry

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FLANGEA, Corina, Catalin SCHIOPU, Eugen SISU, Alina SERB, Michael PRZYBYLSKI, Daniela G. SEIDLER, Alina D. ZAMFIR, 2009. Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry. In: Analytical and Bioanalytical Chemistry. 395(8), pp. 2489-2498. ISSN 1618-2642. eISSN 1618-2650. Available under: doi: 10.1007/s00216-009-3167-0

@article{Flangea2009Deter-9604, title={Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry}, year={2009}, doi={10.1007/s00216-009-3167-0}, number={8}, volume={395}, issn={1618-2642}, journal={Analytical and Bioanalytical Chemistry}, pages={2489--2498}, author={Flangea, Corina and Schiopu, Catalin and Sisu, Eugen and Serb, Alina and Przybylski, Michael and Seidler, Daniela G. and Zamfir, Alina D.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/9604"> <dc:contributor>Flangea, Corina</dc:contributor> <dc:contributor>Schiopu, Catalin</dc:contributor> <dc:creator>Zamfir, Alina D.</dc:creator> <dc:creator>Sisu, Eugen</dc:creator> <dc:language>eng</dc:language> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:creator>Przybylski, Michael</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Zamfir, Alina D.</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:13:05Z</dc:date> <dc:format>application/pdf</dc:format> <dc:contributor>Przybylski, Michael</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Schiopu, Catalin</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:bibliographicCitation>First publ. in: Analytical and Bioanalytical Chemistry, 395 (2009), 8, pp. 2489-2498</dcterms:bibliographicCitation> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:13:05Z</dcterms:available> <dcterms:title>Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry</dcterms:title> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:creator>Seidler, Daniela G.</dc:creator> <dc:creator>Serb, Alina</dc:creator> <dc:creator>Flangea, Corina</dc:creator> <dc:contributor>Sisu, Eugen</dc:contributor> <dc:contributor>Serb, Alina</dc:contributor> <dcterms:issued>2009</dcterms:issued> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9604/1/Determination_Flangea.pdf"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9604/1/Determination_Flangea.pdf"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/9604"/> <dcterms:abstract xml:lang="eng">Chondroitin sulfate (CS) and dermatan sulfate (DS) glycosaminoglycans display variability of sulfation in their constituent disaccharide repeats during chain elongation. Since a large proportion of the extracellular matrix of the central nervous system (CNS) is composed of proteoglycans, CS/DS disaccharide degree and profile of sulfation play important roles in the functional diversity of neurons, brain development, and some of its pathological states. To investigate the sulfation pattern of CS/DS structures expressed in CNS, we introduced here a novel method based on an advanced system encompassing fully automated chip nanoelectrospray ionization (nanoESI) in the negative ion mode and high capacity ion trap multistage mass spectrometry (MS2 MS3) by collision-induced dissociation (CID). This method, introduced here for the first time in glycomics of brain glycosaminoglycans, was particularly applied to structural investigation of disaccharides obtained by β-elimination and digestion with chondroitin B and AC I lyase of hybrid CS/DS chains from wild-type mouse brain. Screening in the chip-MS mode of DS disaccharide fraction resulting after depolymerization with chondroitin B lyase revealed molecular ions assigned to monosulfated disaccharide species having a composition of 4,5-Δ-[IdoA-GalNAc]. By optimized CID MS2 MS3, fragment ions supporting the localization of sulfate ester group at C4 within GalNAc were produced. Chip ESI MS profiling of CS disaccharide fraction obtained by depolymerization of the same CS/DS chain using chondroitin AC I lyase indicated the occurrence of mono- and bisulfated 4,5-Δ-[GlcA-GalNAc]. The site of oversulfation was determined by MS2 MS3, which provided sequence patterns consistent with a rare GlcA-3-sulfate GalNAc-6-sulfate structural motif.</dcterms:abstract> <dc:contributor>Seidler, Daniela G.</dc:contributor> <dc:rights>deposit-license</dc:rights> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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