Predictable suppression of gene expression by 5'-UTR-based RNA quadruplexes


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HALDER, Kangkan, Markus WIELAND, Jörg Steffen HARTIG, 2009. Predictable suppression of gene expression by 5'-UTR-based RNA quadruplexes. In: Nucleic Acids Research. 37(20), pp. 6811-6817. ISSN 0305-1048. eISSN 1362-4962. Available under: doi: 10.1093/nar/gkp696

@article{Halder2009Predi-9552, title={Predictable suppression of gene expression by 5'-UTR-based RNA quadruplexes}, year={2009}, doi={10.1093/nar/gkp696}, number={20}, volume={37}, issn={0305-1048}, journal={Nucleic Acids Research}, pages={6811--6817}, author={Halder, Kangkan and Wieland, Markus and Hartig, Jörg Steffen} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dcterms:rights rdf:resource=""/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Wieland, Markus</dc:creator> <dspace:isPartOfCollection rdf:resource=""/> <dc:contributor>Wieland, Markus</dc:contributor> <dc:language>eng</dc:language> <bibo:uri rdf:resource=""/> <dcterms:isPartOf rdf:resource=""/> <dspace:hasBitstream rdf:resource=""/> <dcterms:available rdf:datatype="">2011-03-24T18:12:42Z</dcterms:available> <dc:contributor>Halder, Kangkan</dc:contributor> <dcterms:issued>2009</dcterms:issued> <dc:format>application/pdf</dc:format> <dc:rights>terms-of-use</dc:rights> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Halder, Kangkan</dc:creator> <dcterms:abstract>Four-stranded DNA and RNA quadruplexes or G4 motifs are non-B DNA conformations that are presumed to form in vivo, although only few explicit evidence has been reported. Using bioinformatics the presence of putative DNA G-quadruplexes within critical promoter regions has been demonstrated and a regulatory role in transcription has been suspected. However, in genomic DNA the presence of the complementary strand interferes with the potential to form a quadruplex motif. Contrarily RNA G4 motifs have no such limitation and consequently strong interference with gene expression is suspected. Nevertheless, experimental evidence is scarce. Here we show a well-defined structure-function relationship of synthetic quadruplex sequences in 5'-UTRs in multiple mammalian cell-lines. We establish a universal 'translational suppressor' effect of these motifs on gene expression at the translational level and show for the first time that specific features such as loop-length and the number of 'GGG'-repeats further determine the suppressive impact. Moreover, a consistent and predictable repression of gene expression is observed for naturally occurring RNA G4 motifs, augmenting the functional relevance of these unusual nucleic acid structures.</dcterms:abstract> <dc:creator>Hartig, Jörg Steffen</dc:creator> <dc:contributor>Hartig, Jörg Steffen</dc:contributor> <dcterms:title>Predictable suppression of gene expression by 5'-UTR-based RNA quadruplexes</dcterms:title> <dcterms:hasPart rdf:resource=""/> <dcterms:bibliographicCitation>First publ. in: Nucleic Acids Research 37 (2009), 20, pp. 6811-6817</dcterms:bibliographicCitation> <dc:date rdf:datatype="">2011-03-24T18:12:42Z</dc:date> </rdf:Description> </rdf:RDF>

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