Der humane MCM4-Genpromoter - eine DNA-Binderegion für Replikationsfaktoren

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LADENBURGER, Eva-Maria, 2002. Der humane MCM4-Genpromoter - eine DNA-Binderegion für Replikationsfaktoren

@phdthesis{Ladenburger2002human-8822, title={Der humane MCM4-Genpromoter - eine DNA-Binderegion für Replikationsfaktoren}, year={2002}, author={Ladenburger, Eva-Maria}, address={Konstanz}, school={Universität Konstanz} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8822"> <dcterms:title>Der humane MCM4-Genpromoter - eine DNA-Binderegion für Replikationsfaktoren</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:46:43Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:46:43Z</dc:date> <dc:contributor>Ladenburger, Eva-Maria</dc:contributor> <dcterms:abstract xml:lang="eng">Minichromosome maintenance proteins (Mcms) and components of the origin recognition complex (Orc1p - Orc6p) have been detected in all eukaryotes and perform important functions in regulating genome duplication. In this work protein-DNA interactions were studied at a region containing the human MCM4-promoter, located between two divergently transcribed genes MCM4 and PRKDC. Performing nuclease protection experiments six protein binding sites were determined. Four of these binding sites are rich in GC base pairs and could promote the binding of transcription factors Sp1 and AP2. Two more distal sites contain a high percentage of AT base pairs and are not related to the known factor binding sites in databanks. In this genomic region a binding site for human Orc proteins 1 and 2 was identified. For this purpose, chromatin immunoprecipitations were combined with quantitative real-time PCR. Sequences corresponding to the intergenic region are highly abundant in a fraction of nascent DNA strands, strongly suggesting that this region harbors not only the binding sites for Orc1 protein and Orc2 protein, but also serves as an origin of bidirectional DNA replication. This is the first binding region identified in the human genome and, at least in this particular region, ORC could mark the start site for DNA replication in the human genome.</dcterms:abstract> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:rights>deposit-license</dc:rights> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8822"/> <dc:creator>Ladenburger, Eva-Maria</dc:creator> <dc:language>deu</dc:language> <dcterms:issued>2002</dcterms:issued> <dcterms:alternative>The human MCM4-genpromoter - a DNA-binding site for replication factors</dcterms:alternative> <dc:format>application/pdf</dc:format> </rdf:Description> </rdf:RDF>

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