KOPS - Das Institutionelle Repositorium der Universität Konstanz

A BAC library for the goldfish Carassius auratus auratus (Cyprinidae, Cypriniformes)

A BAC library for the goldfish Carassius auratus auratus (Cyprinidae, Cypriniformes)

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:c0f7ef7d47a2ff24c9b910ec2847ad82

LUO, Jing, Michael LANG, Walter SALZBURGER, Nicol SIEGEL, Kai N. STÖLTING, Axel MEYER, 2006. A BAC library for the goldfish Carassius auratus auratus (Cyprinidae, Cypriniformes). In: Journal of experimental zoology / Part B, Molecular and developmental evolution. 306 B(Part B), pp. 567-574. ISSN 1552-5007. eISSN 1552-5015

@article{Luo2006libra-8791, title={A BAC library for the goldfish Carassius auratus auratus (Cyprinidae, Cypriniformes)}, year={2006}, doi={10.1002/jez.b.21112}, number={Part B}, volume={306 B}, issn={1552-5007}, journal={Journal of experimental zoology / Part B, Molecular and developmental evolution}, pages={567--574}, author={Luo, Jing and Lang, Michael and Salzburger, Walter and Siegel, Nicol and Stölting, Kai N. and Meyer, Axel} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8791"> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8791"/> <dc:creator>Siegel, Nicol</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:format>application/pdf</dc:format> <dc:contributor>Meyer, Axel</dc:contributor> <dc:creator>Stölting, Kai N.</dc:creator> <dc:contributor>Lang, Michael</dc:contributor> <dcterms:issued>2006</dcterms:issued> <dc:creator>Meyer, Axel</dc:creator> <dcterms:abstract xml:lang="eng">A goldfish (Carassius auratus auratus) bacterial artificial chromosome genomic library (BAC library) was constructed from one aquarium-bred male specimen (tetraploid, 4n5100, genome size53.52 pg/cell). The library consists of 128,352 positive clones with an average insert size of 150.4 kb, covering the genome 11-fold. All clones were spotted onto nylon filters and thus are available for screening of genomic regions of interest, such as candidate genes, gene families, or large-sized syntenic DNA regions of cyprinid species. Preliminary screens with two genes were conducted with hybridizing probes to the genes RAG1 and lgi1. RAG1 is a single-copy gene in zebrafish and is duplicated in C. a. auratus. We found a very close correlation between the number of positive BAC clones and the expected library coverage. Two copies of lgi1 were found in zebrafish. We have detected four different copies in C. a. auratus, not in the expected abundance, which indicates some variation in the coverage of the BAC library. The preliminary screens indicate that many duplicated genes that resulted from the ancient fish-specific genome duplication persist in the tetraploid goldfish genome. Hence, the BAC library will provide a useful resource for the future work on comparative genomics, polyploidy, diploidization, and evolutionary genomics in fishes.</dcterms:abstract> <dc:creator>Lang, Michael</dc:creator> <dcterms:title>A BAC library for the goldfish Carassius auratus auratus (Cyprinidae, Cypriniformes)</dcterms:title> <dc:creator>Salzburger, Walter</dc:creator> <dc:contributor>Siegel, Nicol</dc:contributor> <dc:contributor>Luo, Jing</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:46:29Z</dc:date> <dc:contributor>Stölting, Kai N.</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Luo, Jing</dc:creator> <dc:contributor>Salzburger, Walter</dc:contributor> <dc:rights>deposit-license</dc:rights> <dcterms:bibliographicCitation>First publ. in: Journal of experimental zoology / Part B, Molecular and developmental evolution, 306B (2006), pp. 567 574</dcterms:bibliographicCitation> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:46:29Z</dcterms:available> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

a_bac_library_for_the_goldfish_carassius_auratus_auratus_2006.pdf 176

Das Dokument erscheint in:

deposit-license Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: deposit-license

KOPS Suche


Stöbern

Mein Benutzerkonto