Energetic aspects of malate and lactate fermentation by Acetobacterium malicum


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STROHHÄCKER, Joachim, Bernhard SCHINK, 1991. Energetic aspects of malate and lactate fermentation by Acetobacterium malicum. In: FEMS Microbiology Letters. 90(1), pp. 83-87. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.1991.tb05130.x

@article{Strohhacker1991Energ-8774, title={Energetic aspects of malate and lactate fermentation by Acetobacterium malicum}, year={1991}, doi={10.1111/j.1574-6968.1991.tb05130.x}, number={1}, volume={90}, journal={FEMS Microbiology Letters}, pages={83--87}, author={Strohhäcker, Joachim and Schink, Bernhard} }

Strohhäcker, Joachim eng Strohhäcker, Joachim Acetobacterium malicum ferments L-malate and L-lactate to acetate (and CO.,) as sole fermentation product. Molar growth yieIds we, re 14.5 g and 6.8 g dry cell matter, respectively. With a different Acetobacterium sp. strain, similar results were obtained. No oxaloacetate-decarboxylating enzyme activity was detected in cell-free extracts.<br />u-Malate was oxidized by an unusual NAD-dependent, oxygen-sensitive malic enzyme. No oxatoacetate decarboxylase or malate dehydrogenase activity was found. L-Lactate was oxidized to pyruvate by an NAD(P)-independent enzyme which was partly membrane-associated. Pyruvate conversion to acetate proceeded via pyruvate synthase, phosphate ace,~yltransferase, and acetate kinase. A dichlorophenol indophenol-dependent NADH dehydrogenase was found at high activity.<br />Cytochromes or quinones were not detected. These results, seen against the background of thermodynamical considerations, provide evidence that the difference in growth yields between malate and lactate utilization is due to the difference of redox potentials at which electrons are released during oxidative pyruvate formation from either substrate, and that A. malicum forms ATP by electron transport phosphoryration between NAD and a carrier with a redox potential close to that of quinones. 2011-03-24T17:46:20Z Schink, Bernhard application/pdf terms-of-use Schink, Bernhard Energetic aspects of malate and lactate fermentation by Acetobacterium malicum First publ. in: FEMS Microbiology Letters 90 (1991), pp. 83-88 2011-03-24T17:46:20Z 1991

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