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Entrance Port for Na+ and K+ Ions on Na+,K+-ATPase in the Cytoplasmic loop between trans-membrane segments M6 and M7 of the α subunit : proximity of the cytoplasmic segment of the β subunit

Entrance Port for Na+ and K+ Ions on Na+,K+-ATPase in the Cytoplasmic loop between trans-membrane segments M6 and M7 of the α subunit : proximity of the cytoplasmic segment of the β subunit

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SHAINSKAYA, Alla, Anne SCHNEEBERGER, Hans-Jürgen APELL, Steven J. D. KARLISH, 2000. Entrance Port for Na+ and K+ Ions on Na+,K+-ATPase in the Cytoplasmic loop between trans-membrane segments M6 and M7 of the α subunit : proximity of the cytoplasmic segment of the β subunit. In: The Journal of Biological Chemistry. 275(21), pp. 2019-2028. ISSN 0021-9258. eISSN 1083-351X

@article{Shainskaya2000Entra-8757, title={Entrance Port for Na+ and K+ Ions on Na+,K+-ATPase in the Cytoplasmic loop between trans-membrane segments M6 and M7 of the α subunit : proximity of the cytoplasmic segment of the β subunit}, year={2000}, doi={10.1074/jbc.275.3.2019}, number={21}, volume={275}, issn={0021-9258}, journal={The Journal of Biological Chemistry}, pages={2019--2028}, author={Shainskaya, Alla and Schneeberger, Anne and Apell, Hans-Jürgen and Karlish, Steven J. D.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8757"> <dc:creator>Karlish, Steven J. D.</dc:creator> <dc:creator>Apell, Hans-Jürgen</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:46:13Z</dcterms:available> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8757"/> <dc:contributor>Schneeberger, Anne</dc:contributor> <dcterms:title>Entrance Port for Na+ and K+ Ions on Na+,K+-ATPase in the Cytoplasmic loop between trans-membrane segments M6 and M7 of the α subunit : proximity of the cytoplasmic segment of the β subunit</dcterms:title> <dcterms:abstract xml:lang="eng">Based on the following observations we propose that the cytoplasmic loop between trans-membrane segments M6 and M7 (L6/7) of the α subunit of Na+,K+-ATPase acts as an entrance port for Na+ and K+ ions. 1) In defined conditions chymotrypsin specifically cleaves L6/7 in the M5/M6 fragment of 19-kDa membranes, produced by extensive proteolysis of Na+,K+-ATPase, and in parallel inactivates Rb1 occlusion. 2) Dissociation of the M5/M6 fragment from 19-kDa membranes is prevented either by occluded cations or by competitive antagonists such as Ca²+, Mg²+, La³+, p-xylylene bisguanidinium and m-xylylene bisguanidinium, or 1-bromo- 2,4,6-tris(methylisothiouronium)benzene and 1,3-dibromo-2,4,6-tris (methylisothiouronium)benzene (Br2-TITU³1). 3) Ca²1 ions raise electrophoretic mobility of the M5/M6 fragment but not that of the other fragments of the a subunit. It appears that negatively charged residues in L6/7 recognize either Na+ or K+ ions or the competitive cation antagonists. Na+ and K+ ions are then occluded within trans-membrane segments and can be transported, whereas the cation antagonists are not occluded and block transport at the entrance port. The cytoplasmic segment of the β subunit appears to be close to or contributes to the entrance port, as inferred from the following observations. 1) Specific chymotryptic cleavage of the 16-kDa fragment of the β subunit to 15-kDa at 20°C (Shainskaya, A., and Karlish, S. J. D. (1996) J. Biol. Chem. 271, 10309 10316) markedly reduces affinity for Br2-TITU³+ and for Na+ ions, detected by Na+ occlusion assays or electrogenic Na+ binding, whereas Rb+ occlusion is unchanged. 2) Na+ ions specifically protect the 16-kDa fragment against this chymotryptic cleavage.</dcterms:abstract> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:issued>2000</dcterms:issued> <dc:creator>Schneeberger, Anne</dc:creator> <dc:contributor>Karlish, Steven J. D.</dc:contributor> <dc:language>eng</dc:language> <dc:format>application/pdf</dc:format> <dc:creator>Shainskaya, Alla</dc:creator> <dcterms:bibliographicCitation>First publ. in: The Journal of Biological Chemistry 275 (2000), 21, pp. 2019-2028</dcterms:bibliographicCitation> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:46:13Z</dc:date> <dc:contributor>Apell, Hans-Jürgen</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:contributor>Shainskaya, Alla</dc:contributor> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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