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Highly versatile confocal microscopy system based on a tunable femtosecond Er:fiber source

Highly versatile confocal microscopy system based on a tunable femtosecond Er:fiber source

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TRÄUTLEIN, Daniel, Florian ADLER, Konstantinos MOUTZOURIS, Andreas JEROMIN, Alfred LEITENSTORFER, Elisa MAY, 2008. Highly versatile confocal microscopy system based on a tunable femtosecond Er:fiber source. In: Journal of Biophotonics. 1(1), pp. 53-61. ISSN 1864-063X. eISSN 1864-0648

@article{Trautlein2008-03Highl-874, title={Highly versatile confocal microscopy system based on a tunable femtosecond Er:fiber source}, year={2008}, doi={10.1002/jbio.200710019}, number={1}, volume={1}, issn={1864-063X}, journal={Journal of Biophotonics}, pages={53--61}, author={Träutlein, Daniel and Adler, Florian and Moutzouris, Konstantinos and Jeromin, Andreas and Leitenstorfer, Alfred and May, Elisa} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/874"> <dc:creator>Träutlein, Daniel</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:contributor>May, Elisa</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-22T17:51:39Z</dc:date> <dc:contributor>Jeromin, Andreas</dc:contributor> <dc:contributor>Träutlein, Daniel</dc:contributor> <dcterms:abstract xml:lang="eng">The performance of a confocal microscopy setup based on a single femtosecond fiber system is explored over a broad range of pump wavelengths for both linear and nonlinear imaging techniques. First, the-benefits of a laser source in linear fluorescence excitation that is continuously tunable over most of the visible, spectrum are demonstrated. The influences of subpicosecond pulse durations on the bleaching behavior of typical fluorophores are discussed. We then utilize the tunable near-infrared output of the femto-second system in connection with a specially designed prism compressor for dispersion control. Pulses as short as 33 fs are measured in the confocal region. As a consequence, 2 mW of average power are sufficient for two-photon microscopy in an organotypic sample from the, mouse brain. This result shows great prospect for deep-tissue imaging in the optimum transparency window around 1100 nm. In a third experiment, we prove that out, compact setup is powerful enough to exploit even higher-order non-linearities such as three-photon absorption that we use to induce spatially localized photodamage in DNA.</dcterms:abstract> <dc:creator>May, Elisa</dc:creator> <dcterms:bibliographicCitation>First publ. in: Journal of Biophotonics ; 1 (2008), 1. - pp. 53-61</dcterms:bibliographicCitation> <dcterms:title>Highly versatile confocal microscopy system based on a tunable femtosecond Er:fiber source</dcterms:title> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/874"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-22T17:51:39Z</dcterms:available> <dc:creator>Leitenstorfer, Alfred</dc:creator> <dc:contributor>Leitenstorfer, Alfred</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Jeromin, Andreas</dc:creator> <dc:contributor>Adler, Florian</dc:contributor> <dc:contributor>Moutzouris, Konstantinos</dc:contributor> <dc:creator>Moutzouris, Konstantinos</dc:creator> <dc:creator>Adler, Florian</dc:creator> <dcterms:issued>2008-03</dcterms:issued> </rdf:Description> </rdf:RDF>

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Leitensdorfer etal.pdf 193

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