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Bioluminescence emission of bacterial luciferase with 1-deaza-FMN : evidence for the noninvolvement of N(1)-protonated flavin species as emitters

Bioluminescence emission of bacterial luciferase with 1-deaza-FMN : evidence for the noninvolvement of N(1)-protonated flavin species as emitters

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KURFÜRST, Manfred, Peter MACHEROUX, Sandro GHISLA, J. Woodland HASTINGS, 1989. Bioluminescence emission of bacterial luciferase with 1-deaza-FMN : evidence for the noninvolvement of N(1)-protonated flavin species as emitters. In: European Journal of Biochemistry. 181(2), pp. 453-457. ISSN 0014-2956. eISSN 1432-1033

@article{Kurfurst1989Biolu-8713, title={Bioluminescence emission of bacterial luciferase with 1-deaza-FMN : evidence for the noninvolvement of N(1)-protonated flavin species as emitters}, year={1989}, doi={10.1111/j.1432-1033.1989.tb14746.x}, number={2}, volume={181}, issn={0014-2956}, journal={European Journal of Biochemistry}, pages={453--457}, author={Kurfürst, Manfred and Macheroux, Peter and Ghisla, Sandro and Hastings, J. Woodland} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8713"> <dc:creator>Hastings, J. Woodland</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:53Z</dcterms:available> <dc:contributor>Ghisla, Sandro</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Ghisla, Sandro</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8713"/> <dc:rights>deposit-license</dc:rights> <dc:creator>Macheroux, Peter</dc:creator> <dc:creator>Kurfürst, Manfred</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:53Z</dc:date> <dcterms:bibliographicCitation>First publ. in: European Journal of Biochemistry 181 (1989), 2, pp. 453-457</dcterms:bibliographicCitation> <dcterms:title>Bioluminescence emission of bacterial luciferase with 1-deaza-FMN : evidence for the noninvolvement of N(1)-protonated flavin species as emitters</dcterms:title> <dc:contributor>Hastings, J. Woodland</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Macheroux, Peter</dc:contributor> <dcterms:issued>1989</dcterms:issued> <dcterms:abstract xml:lang="eng">The reaction of reduced 1-d-FMN with oxygen and decanal results in bioluminescence with kinetic and spectral properties similar to those of the reaction with FMNH2, even though the spectral (absorbance, fluorescence) and chemical properties of the oxidized forms differ greatly. This emission, which is about 10 15% as efficient as with FMNH2, is postulated to involve the intermediacy of the corresponding 4a-hydroperoxide, the fluorescence of which occurred transiently. The N(1) protonated species had been proposed as the emitter in the reaction with FMNH2, but the 1-deaza analog cannot be protonated at the corresponding position, thus excluding this possibility.</dcterms:abstract> <dc:contributor>Kurfürst, Manfred</dc:contributor> <dc:format>application/pdf</dc:format> </rdf:Description> </rdf:RDF>

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