Bioluminescence emission of bacterial luciferase with 1-deaza-FMN : evidence for the noninvolvement of N(1)-protonated flavin species as emitters
| Publikationstyp: | Zeitschriftenartikel |
| URI (zitierfähiger Link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-55424 |
| Autor/innen: | Kurfürst, Manfred; Macheroux, Peter; Ghisla, Sandro; Hastings, J. Woodland |
| Erscheinungsjahr: | 1989 |
| Erschienen in: | European Journal of Biochemistry ; 181 (1989), 2. - S. 453-457. - ISSN 0014-2956. - eISSN 1432-1033 |
| DOI (zitierfähiger Link): | https://dx.doi.org/10.1111/j.1432-1033.1989.tb14746.x |
| Zusammenfassung: |
The reaction of reduced 1-d-FMN with oxygen and decanal results in bioluminescence with kinetic and spectral properties similar to those of the reaction with FMNH2, even though the spectral (absorbance, fluorescence) and chemical properties of the oxidized forms differ greatly. This emission, which is about 10 15% as efficient as with FMNH2, is postulated to involve the intermediacy of the corresponding 4a-hydroperoxide, the fluorescence of which occurred transiently. The N(1) protonated species had been proposed as the emitter in the reaction with FMNH2, but the 1-deaza analog cannot be protonated at the corresponding position, thus excluding this possibility.
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| Fachgebiet (DDC): | 570 Biowissenschaften, Biologie |
| Link zur Lizenz: | Nutzungsbedingungen |
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KURFÜRST, Manfred, Peter MACHEROUX, Sandro GHISLA, J. Woodland HASTINGS, 1989. Bioluminescence emission of bacterial luciferase with 1-deaza-FMN : evidence for the noninvolvement of N(1)-protonated flavin species as emitters. In: European Journal of Biochemistry. 181(2), pp. 453-457. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1111/j.1432-1033.1989.tb14746.x
@article{Kurfurst1989Biolu-8713, title={Bioluminescence emission of bacterial luciferase with 1-deaza-FMN : evidence for the noninvolvement of N(1)-protonated flavin species as emitters}, year={1989}, doi={10.1111/j.1432-1033.1989.tb14746.x}, number={2}, volume={181}, issn={0014-2956}, journal={European Journal of Biochemistry}, pages={453--457}, author={Kurfürst, Manfred and Macheroux, Peter and Ghisla, Sandro and Hastings, J. Woodland} }
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