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Acetate oxidation through a modified citric acid cycle in Propionibacterium freudenreichii

Acetate oxidation through a modified citric acid cycle in Propionibacterium freudenreichii

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BECK, Sven, Bernhard SCHINK, 1995. Acetate oxidation through a modified citric acid cycle in Propionibacterium freudenreichii. In: Archives of Microbiology. 163(3), pp. 182-187. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00305351

@article{Beck1995Aceta-8710, title={Acetate oxidation through a modified citric acid cycle in Propionibacterium freudenreichii}, year={1995}, doi={10.1007/BF00305351}, number={3}, volume={163}, issn={0302-8933}, journal={Archives of Microbiology}, pages={182--187}, author={Beck, Sven and Schink, Bernhard} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8710"> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:51Z</dcterms:available> <dcterms:abstract xml:lang="eng">Propionibacterium freudenreichii strain DSM 20271 was grown in a mineral medium containing 0.1% (w/v) yeast extract. Acetate was oxidized by growing cells with potassium hexacyanofen-ate as electron acceptot, which was oxidized by a three-electrode poised-potential system at a redox potential of +510 mV. Growth with acetate under these conditions followed linear rather than exponential kinetics, whereas growth with other substrates such as lactate under the same conditions was exponential. Cell-free extracts of P. freudenreichii cells grown with acetate contained all enzymes of the classical citric acid cycle except 2-oxoglutarate-oxidizing activity. No activity of anaplerotic reactions such as isocitrate lyase or malate synthase was found. Instead, moderate activities of glutamate decarboxylase, 4-aminobutyrate:2-oxoglutarate aminotransferase, and succinate semialdehyde dehydrogenase were detected. In short-term radiolabeling experiments with U-~4C-acetate, 4-aminobutyrate was identified as a major early intermediate in acetate oxidation by these cells. These findings allow the construction of a modified citric acid cycle that compensates the lack of 2-oxoglutarate dehydrogenase by a subcycle through glutamate, 4-aminobutyrate, and succinate semialdehyde. Lack of anaplerotic reactions explains subexponential growth kinetics during growth with acetate.</dcterms:abstract> <dc:contributor>Beck, Sven</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:51Z</dc:date> <dc:contributor>Schink, Bernhard</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:language>eng</dc:language> <dc:creator>Beck, Sven</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8710/1/Acetate_oxydation_through_a_modified_1995.pdf"/> <dcterms:issued>1995</dcterms:issued> <dc:format>application/pdf</dc:format> <dcterms:title>Acetate oxidation through a modified citric acid cycle in Propionibacterium freudenreichii</dcterms:title> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 163 (1995), 3, pp. 182-187</dcterms:bibliographicCitation> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8710/1/Acetate_oxydation_through_a_modified_1995.pdf"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8710"/> <dc:creator>Schink, Bernhard</dc:creator> <dc:rights>deposit-license</dc:rights> </rdf:Description> </rdf:RDF>

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