KOPS - Das Institutionelle Repositorium der Universität Konstanz

Purification and some properties of (1R,2S)-1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase from Comamonas testosteroni T-2

Purification and some properties of (1R,2S)-1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase from Comamonas testosteroni T-2

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:b7db9edc3b93283d13b3c9ed8ed7df6e

SALLER, Elisabeth, Heike LAUE, Hans Rudolf SCHLÄFLI OPPENBERG, Alasdair M. COOK, 1995. Purification and some properties of (1R,2S)-1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase from Comamonas testosteroni T-2. In: FEMS Microbiology Letters. 130(1), pp. 97-102. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.1995.tb07705.x

@article{Saller1995Purif-8655, title={Purification and some properties of (1R,2S)-1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase from Comamonas testosteroni T-2}, year={1995}, doi={10.1111/j.1574-6968.1995.tb07705.x}, number={1}, volume={130}, issn={0378-1097}, journal={FEMS Microbiology Letters}, pages={97--102}, author={Saller, Elisabeth and Laue, Heike and Schläfli Oppenberg, Hans Rudolf and Cook, Alasdair M.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8655"> <dc:contributor>Laue, Heike</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Saller, Elisabeth</dc:contributor> <dcterms:abstract xml:lang="eng">AbstractInducible (1R,2S)-1,2-dihydroxy-3,5-cyclohexadiene-l,4-dicarboxylate (diene-diol) dehydrogenase was found in extracts of Comamonas testosteroni T-2 grown in p-toluate-or terephthalate-salts medium and it was purified using anion exchange, hydrophobic interaction and gel filtration chromatography. The enzyme is a homodimer with subunit Mr 39000. It had a specific activity of 500 mkat/kg of protein and was activated by the addition of Fe2+. The dehydrogenase converted 1 mol diene-diol and 1 mol NAD+ to 1 mol protocatechuic acid, 1 mol NADH and 1 mol CO2. Apparent Km-values of 43 µM (NAD+) and about 90 µM (diene-diol) were determined. The hydride ion was transferred to the si face of NAD+.</dcterms:abstract> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8655/1/Purificationandsomepropertiesof.pdf"/> <dcterms:title>Purification and some properties of (1R,2S)-1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase from Comamonas testosteroni T-2</dcterms:title> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Saller, Elisabeth</dc:creator> <dc:contributor>Schläfli Oppenberg, Hans Rudolf</dc:contributor> <dc:creator>Cook, Alasdair M.</dc:creator> <dcterms:issued>1995</dcterms:issued> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:27Z</dcterms:available> <dcterms:bibliographicCitation>First publ. in: FEMS Microbiology Letters 130 (1995), 1, pp. 97-102</dcterms:bibliographicCitation> <dc:creator>Laue, Heike</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:27Z</dc:date> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Schläfli Oppenberg, Hans Rudolf</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8655/1/Purificationandsomepropertiesof.pdf"/> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dc:format>application/pdf</dc:format> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8655"/> <dc:language>eng</dc:language> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:rights>deposit-license</dc:rights> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Purificationandsomepropertiesof.pdf 95

Das Dokument erscheint in:

deposit-license Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: deposit-license

KOPS Suche


Stöbern

Mein Benutzerkonto