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Functional Analysis of D-Alanylation of Lipoteichoic Acid in the Probiotic Strain Lactobacillus rhamnosus GG

Functional Analysis of D-Alanylation of Lipoteichoic Acid in the Probiotic Strain Lactobacillus rhamnosus GG

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VÉLEZ, Mónica Perea, Tine L. A. VERHOEVEN, Christian DRAING, Sonja von AULOCK, Markus PFITZENMAIER, Armin GEYER, Ivo LAMBRICHTS, Corinne GRANGETTE, Bruno POT, Jos VANDERLEYDEN, Sigrid C. J. de KEERSMAECKER, 2007. Functional Analysis of D-Alanylation of Lipoteichoic Acid in the Probiotic Strain Lactobacillus rhamnosus GG. In: Applied and environmental microbiology. 73(11), pp. 3595-3604. ISSN 0099-2240

@article{Velez2007Funct-8623, title={Functional Analysis of D-Alanylation of Lipoteichoic Acid in the Probiotic Strain Lactobacillus rhamnosus GG}, year={2007}, doi={10.1128/AEM.02083-06}, number={11}, volume={73}, issn={0099-2240}, journal={Applied and environmental microbiology}, pages={3595--3604}, author={Vélez, Mónica Perea and Verhoeven, Tine L. A. and Draing, Christian and Aulock, Sonja von and Pfitzenmaier, Markus and Geyer, Armin and Lambrichts, Ivo and Grangette, Corinne and Pot, Bruno and Vanderleyden, Jos and Keersmaecker, Sigrid C. J. de} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8623"> <dc:contributor>Vanderleyden, Jos</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Applied and environmental microbiology 73 (2007), 11, pp. 3595-3604</dcterms:bibliographicCitation> <dc:creator>Lambrichts, Ivo</dc:creator> <dc:contributor>Geyer, Armin</dc:contributor> <dc:contributor>Pot, Bruno</dc:contributor> <dc:creator>Vanderleyden, Jos</dc:creator> <dc:contributor>Keersmaecker, Sigrid C. J. de</dc:contributor> <dc:contributor>Aulock, Sonja von</dc:contributor> <dcterms:abstract xml:lang="eng">Lipoteichoic acid (LTA) is a macroamphiphile molecule which performs several functions in gram-positive bacteria, such as maintenance of cell wall homeostasis. D-Alanylation of LTA requires the proteins encoded by the dlt operon, and this process is directly related to the charge properties of this polymer strongly contributing to its function. The insertional inactivation of dltD of the probiotic strain Lactobacillus rhamnosus GG (ATCC 53103) resulted in the complete absence of D-alanyl esters in the LTA as confirmed by nuclear magnetic resonance analysis. This was reflected in modifications of the bacterial cell surface properties. The dltD strain showed 2.4-fold-increased cell length, a low survival capacity in response to gastric juice challenge, an increased sensitivity to human beta-defensin-2, an increased rate of autolysis, an increased capacity to initiate growth in the presence of an anionic detergent, and a decreased capacity to initiate growth in the presence of cationic peptides compared to wild-type results. However, in vitro experiments revealed no major differences for adhesion to human intestinal epithelial cells, biofilm formation, and immunomodulation. These properties are considered to be important for probiotics. The role of the dlt operon in lactobacilli is discussed in view of these results.</dcterms:abstract> <dc:contributor>Lambrichts, Ivo</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:creator>Keersmaecker, Sigrid C. J. de</dc:creator> <dc:contributor>Verhoeven, Tine L. A.</dc:contributor> <dc:creator>Pot, Bruno</dc:creator> <dc:creator>Verhoeven, Tine L. A.</dc:creator> <dc:creator>Aulock, Sonja von</dc:creator> <dcterms:title>Functional Analysis of D-Alanylation of Lipoteichoic Acid in the Probiotic Strain Lactobacillus rhamnosus GG</dcterms:title> <dc:creator>Geyer, Armin</dc:creator> <dc:contributor>Grangette, Corinne</dc:contributor> <dc:creator>Draing, Christian</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:12Z</dc:date> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8623"/> <dcterms:issued>2007</dcterms:issued> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:12Z</dcterms:available> <dc:creator>Pfitzenmaier, Markus</dc:creator> <dc:format>application/pdf</dc:format> <dc:contributor>Draing, Christian</dc:contributor> <dc:creator>Grangette, Corinne</dc:creator> <dc:contributor>Pfitzenmaier, Markus</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Vélez, Mónica Perea</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:creator>Vélez, Mónica Perea</dc:creator> </rdf:Description> </rdf:RDF>

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