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Structural and functional investigations on multi-site metallo enzymes of the biological sulfur cycle

Structural and functional investigations on multi-site metallo enzymes of the biological sulfur cycle

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SCHIFFER, Alexander, 2004. Structural and functional investigations on multi-site metallo enzymes of the biological sulfur cycle [Dissertation]. Konstanz: University of Konstanz

@phdthesis{Schiffer2004Struc-8619, title={Structural and functional investigations on multi-site metallo enzymes of the biological sulfur cycle}, year={2004}, author={Schiffer, Alexander}, address={Konstanz}, school={Universität Konstanz} }

2004 Schiffer, Alexander deposit-license In this Ph.D. thesis the structural, functional and spectroscopic properties of two key enzymes of dissimilatory sulfate reduction were investigated.<br />Crystal structure of APS reductase from Archaeoglobus fulgidus<br />The iron-sulfur flavoenzyme adenylylsulfate (adenosine 5 -phosphosulfate, APS) reductase catalyzes reversibly the reduction of APS to sulfite and AMP. The structure of APS reductase from the hyperthermophilic A. fulgidus in the two-electron reduced state was reported at 1.6 Å resolution (Proc Natl Acad Sci USA. 2002; 99:1836-41).<br />Structure based catalytic mechanism of APS reductase<br />To elucidate the reaction cycle of APS reductase various states of the enzyme along the reaction coordinate were structurally characterized.<br />Biochemical and spectroscopic characterization of sulfite reductase from Archaeoglobus fulgidus<br />Dissimilatory sulfite reductase from the hyperthermophilic archaeon A. fulgidus was isolated and purified under strict exclusion of dioxygen in a N2/H2 (95/5 %) atmosphere.<br />Crystallization and X-ray analysis of sulfite reductase<br />Sulfite reductase from A. fulgidus was crystallized under exclusion of dioxygen using the hanging drop vapor diffusion method. The crystallization was carried out at 18°C using PEG 4000 as precipitant. Structural and functional investigations on multi-site metallo enzymes of the biological sulfur cycle Strukturelle und funktionelle Untersuchungen an Mehrzentren Metalloenzymen des biologischen Schwefelkreislaufs application/pdf 2011-03-24T17:45:10Z Schiffer, Alexander 2011-03-24T17:45:10Z eng

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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