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Characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an Arthrobacter sp.

Characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an Arthrobacter sp.

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SCHOLTZ, Rudolf, Thomas LEISINGER, Franz SUTER, Alasdair M. COOK, 1987. Characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an Arthrobacter sp.. In: Journal of Bacteriology. 169(11), pp. 5016-5021. ISSN 0021-9193. eISSN 1098-5530

@article{Scholtz1987Chara-8613, title={Characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an Arthrobacter sp.}, year={1987}, number={11}, volume={169}, issn={0021-9193}, journal={Journal of Bacteriology}, pages={5016--5021}, author={Scholtz, Rudolf and Leisinger, Thomas and Suter, Franz and Cook, Alasdair M.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8613"> <dc:language>eng</dc:language> <dc:contributor>Leisinger, Thomas</dc:contributor> <dc:creator>Scholtz, Rudolf</dc:creator> <dc:rights>deposit-license</dc:rights> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:creator>Cook, Alasdair M.</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:06Z</dcterms:available> <dc:creator>Suter, Franz</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8613"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:06Z</dc:date> <dcterms:bibliographicCitation>First publ. in: Journal of Bacteriology 169 (1987), 11, pp. 5016-5021</dcterms:bibliographicCitation> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dcterms:issued>1987</dcterms:issued> <dc:format>application/pdf</dc:format> <dc:creator>Leisinger, Thomas</dc:creator> <dc:contributor>Scholtz, Rudolf</dc:contributor> <dc:contributor>Suter, Franz</dc:contributor> <dcterms:abstract xml:lang="eng">1-Chlorohexane halidohydrolase from Arthrobacter sp. strain HA1 was purified to homogeneity by fractional precipitation, ion-exchange chromatography, gel filtration, and high-performance liquid chromatography gel filtration. The enzyme was a monomer with a molecular weight of about 37,000; its amino acid composition and N-terminal sequence were determined. The enzyme had a broad optimum around pH 9.5, a temperature optimum near 50 degrees C, an activation energy of 40 kJ/mol, and a molecular activity of 0.9 kat/mol. The substrate range of the enzyme included at least 50 halogenated compounds. 1-Chloroalkanes (C3 to C10), 1-bromoalkanes (C1 to C9), and 1-iodoalkanes (C1 to C7), but no 1-fluoroalkane, were substrates. Subterminally substituted, branched-chain, and nonsaturated haloalkanes were dehalogenated. Some halogenated aromatic substrates, e.g., bromobenzene and benzyl bromide, were hydrolyzed. Several alpha,omega-dihaloalkanes were subject to double dehalogenation. Thus, 1,2-dibromoethane was hydrolyzed first to 2-bromoethanol and then to 1,2-dihydroxyethane. Crude extracts of strain HA1 were found to contain a debrominase that cleaved bromoalkanes with long alkyl chains.</dcterms:abstract> <dcterms:title>Characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an Arthrobacter sp.</dcterms:title> </rdf:Description> </rdf:RDF>

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