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Quantitative estimation of the surface carbohydrates on the infection structures of rust fungi with enzymes and lectins

Quantitative estimation of the surface carbohydrates on the infection structures of rust fungi with enzymes and lectins

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MENDGEN, Kurt, Martina LANGE, Karin BRETSCHNEIDER, 1985. Quantitative estimation of the surface carbohydrates on the infection structures of rust fungi with enzymes and lectins. In: Archives of Microbiology. 140(4), pp. 307-311. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00446968

@article{Mendgen1985Quant-8603, title={Quantitative estimation of the surface carbohydrates on the infection structures of rust fungi with enzymes and lectins}, year={1985}, doi={10.1007/BF00446968}, number={4}, volume={140}, issn={0302-8933}, journal={Archives of Microbiology}, pages={307--311}, author={Mendgen, Kurt and Lange, Martina and Bretschneider, Karin} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8603"> <dc:creator>Bretschneider, Karin</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8603/1/55_mendgen85.pdf"/> <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 140 (1985), pp. 307-311</dcterms:bibliographicCitation> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Lange, Martina</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:02Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:02Z</dc:date> <dc:format>application/pdf</dc:format> <dcterms:abstract xml:lang="eng">Lectins of Triticum vulgaris (WGA), Concanavalia ensiformis (ConA), Phaseolus vulgaris (PHA), Lotus tetragonolobus (LTA), Araehis hypogaea (PNA), Ricinus communis (RCA I), Griffonia simplicifolia (GSA II) were tested for binding to the infection structures of Puccinia coronata and Uromyces appendiculatus. The enzymes and lectins were labeled with fluorescein, and the fluorescence was measured with a microscope photometer. GSA II and ConA bound to all parts of the two rust fungi to a certain extent. The germ tubes of P. coronata bound at least two times more WGA than did the germ tubes of U. appendicutatus. The substomatal vesicle and the infection hypha of both rust fungi mainly bound the glucanases. Furthermore, the substomatal vesicle of U. appendiculatus bound PHA. No obvious binding with LTA, RCA I and PNA was observed. Binding generally could be inhibited by appropriate haptens. Binding to uredospores generally appeared unspecific. The results indicate that the germ tubes have chitin on their outer surfaces, the appressoria chitin and glucans and the substomatal vesicles and infection hyphae mainly glucans. Compared to P. coronata, U. appendiculatus has more terminal linked glucose residues. Also, U. appendiculatus has N-acetylgalactosamine or a similar sugar on the surface of the substomatal vesicle.</dcterms:abstract> <dcterms:title>Quantitative estimation of the surface carbohydrates on the infection structures of rust fungi with enzymes and lectins</dcterms:title> <dc:language>eng</dc:language> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8603"/> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Mendgen, Kurt</dc:contributor> <dc:creator>Mendgen, Kurt</dc:creator> <dc:rights>deposit-license</dc:rights> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Bretschneider, Karin</dc:contributor> <dc:creator>Lange, Martina</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8603/1/55_mendgen85.pdf"/> <dcterms:issued>1985</dcterms:issued> </rdf:Description> </rdf:RDF>

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