KOPS - The Institutional Repository of the University of Konstanz

Determination of a novel structure by a combination of long-wavelength sulfur phasing and radiation-damage-induced phasing

Determination of a novel structure by a combination of long-wavelength sulfur phasing and radiation-damage-induced phasing

Cite This

Files in this item

Checksum: MD5:53474f38833aceaf2adc0c0a5306e609

WEISS, Manfred S., Gerd MANDER, Reiner HEDDERICH, Kay DIEDERICHS, Ulrich ERMLER, Eberhard WARKENTIN, 2004. Determination of a novel structure by a combination of long-wavelength sulfur phasing and radiation-damage-induced phasing. In: Acta Crystallographica, Section D. 60(4), pp. 686-695. ISSN 0907-4449. Available under: doi: 10.1107/S0907444904003002

@article{Weiss2004Deter-8593, title={Determination of a novel structure by a combination of long-wavelength sulfur phasing and radiation-damage-induced phasing}, year={2004}, doi={10.1107/S0907444904003002}, number={4}, volume={60}, issn={0907-4449}, journal={Acta Crystallographica, Section D}, pages={686--695}, author={Weiss, Manfred S. and Mander, Gerd and Hedderich, Reiner and Diederichs, Kay and Ermler, Ulrich and Warkentin, Eberhard} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8593"> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:57Z</dcterms:available> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Mander, Gerd</dc:creator> <dc:contributor>Hedderich, Reiner</dc:contributor> <dc:creator>Hedderich, Reiner</dc:creator> <dc:creator>Warkentin, Eberhard</dc:creator> <dc:contributor>Diederichs, Kay</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8593/1/Determination_of_a_novel_structure_by_a_combination.pdf"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8593"/> <dc:format>application/pdf</dc:format> <dc:contributor>Ermler, Ulrich</dc:contributor> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dc:contributor>Weiss, Manfred S.</dc:contributor> <dc:contributor>Mander, Gerd</dc:contributor> <dc:creator>Weiss, Manfred S.</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8593/1/Determination_of_a_novel_structure_by_a_combination.pdf"/> <dc:creator>Ermler, Ulrich</dc:creator> <dc:creator>Diederichs, Kay</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:57Z</dc:date> <dc:contributor>Warkentin, Eberhard</dc:contributor> <dc:language>eng</dc:language> <dcterms:issued>2004</dcterms:issued> <dcterms:abstract xml:lang="eng">The structure of the 115 amino-acid residue protein DsvC was determined based on the anomalous scattering provided by the five S atoms present in the structure. By collecting the diffraction data at a wavelength of 1.9 A, the anomalous signal provided by the S atoms was enhanced. However, significant radiation damage occurred during the course of the experiment, which led to differences between different parts of the data set. Only by dividing the total data set into five data sets was it possible to obtain phases; these could then be successfully extended to allow structure determination by the automated model-building program ARP/wARP. A computational correction for the radiation damage was found to significantly improve the success rate in determining the heavy-atom substructure and to improve phasing and refinement statistics.</dcterms:abstract> <dcterms:title>Determination of a novel structure by a combination of long-wavelength sulfur phasing and radiation-damage-induced phasing</dcterms:title> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <dcterms:bibliographicCitation>First publ. in: Acta Crystallographica, Section D 60 (2004), pp. 686-695</dcterms:bibliographicCitation> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

Determination_of_a_novel_structure_by_a_combination.pdf 389

This item appears in the following Collection(s)

Attribution-NonCommercial-NoDerivs 2.0 Generic Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.0 Generic

Search KOPS


Browse

My Account