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Prostaglandin 9-ketoreductase from pig and human kidney : purification, properties and identity with human carbonyl reductase

Prostaglandin 9-ketoreductase from pig and human kidney : purification, properties and identity with human carbonyl reductase

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SCHIEBER, Ardrea, Sandro GHISLA, 1992. Prostaglandin 9-ketoreductase from pig and human kidney : purification, properties and identity with human carbonyl reductase. In: Eicosanoids. 5(Suppl), pp. 37-38

@article{Schieber1992Prost-8591, title={Prostaglandin 9-ketoreductase from pig and human kidney : purification, properties and identity with human carbonyl reductase}, year={1992}, number={Suppl}, volume={5}, journal={Eicosanoids}, pages={37--38}, author={Schieber, Ardrea and Ghisla, Sandro} }

First publ. in: Eicosanoids 5 (1992), Suppl., pp. 37-38 Prostaglandin 9-ketoreductase from pig and human kidney : purification, properties and identity with human carbonyl reductase Schieber, Ardrea Ghisla, Sandro Ghisla, Sandro 2011-03-24T17:44:56Z application/pdf Schieber, Ardrea eng 1992 Prostaglandin 9-ketoreductase has been purified to apparent homogeneity from pig and human kidney with an overall yield of 6%. The enzyme has a molecular mass of 34 kDa, it is present as an active monomer in diluted solution and contains approx. 2 equivalents Zn++/mole enzyme. It is stereoselective for the pro(S) hydrogen of NADPH and reduces the prostaglandin 9-keto group to yield 90% prostaglandin F2α and 8% of the ß-form. An extensive study of the catalytic properties was carried out, which leads ta the conclusion that the enzyme function in vivo is unlikely a catalysis of oxidation/reduction at the prostaglandin 9-position. Five peptides from the pig kidney enzyme were sequenced and compared with the sequence of carbonyl reductase from human placenta. The identity is > 90% and this, together with the immunological cross-reactivity with human brain carbonyl reductase, most strongly suggests that prostaglandin 9-ketoreductase and carbonyl reductase are the same enzyme. terms-of-use 2011-03-24T17:44:56Z

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