@article{Mohamed2003Refil-8529,
  year={2003},
  doi={10.1016/S0143-4160(03)00025-3},
  title={Refilling of cortical calcium stores in Paramecium cells : in situ analysis in correlation with store-operated calcium influx},
  number={1},
  volume={34},
  issn={0143-4160},
  journal={Cell Calcium},
  pages={87--96},
  author={Mohamed, Ihab K. and Husser, Marc R. and Sehring, Ivonne Margarete and Hentschel, Joachim and Hentschel, C. and Plattner, Helmut}
}

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    <dc:creator>Sehring, Ivonne Margarete</dc:creator>
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    <dc:contributor>Sehring, Ivonne Margarete</dc:contributor>
    <dc:contributor>Husser, Marc R.</dc:contributor>
    <dc:contributor>Hentschel, C.</dc:contributor>
    <dc:contributor>Plattner, Helmut</dc:contributor>
    <dc:contributor>Hentschel, Joachim</dc:contributor>
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    <dcterms:title>Refilling of cortical calcium stores in Paramecium cells : in situ analysis in correlation with store-operated calcium influx</dcterms:title>
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    <dc:contributor>Mohamed, Ihab K.</dc:contributor>
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    <dcterms:abstract xml:lang="eng">This is the first thorough study of refilling of a cortical calcium store in a secretory cell after stimulation in which we combined widely different methodologies. Stimulation of dense-core vesicle ( trichocysts ) exocytosis in Paramecium involves a Ca2+-influx  superimposed to Ca2+-release from cortical stores ( alveolar sacs  (ASs)). In quenched-flow experiments, membrane fusion frequency rose with increasing [Ca2+]o in the medium, from 20 25% at [Ca2+]o ≤0.25 μM to 100% at [Ca2+]o between 2 and 10 μM, i.e. close to the range of estimated local intracellular [Ca2+] during membrane fusion. Next, we analyzed Ca2+-specific fluorochrome signals during stimulation under different conditions. Treatment with actin-reactive drugs had no effect on Ca2+-signaling. In double trigger experiments, with BAPTA in the second secretagogue application (BAPTA only for stimulation and analysis), the cortical Ca2+-signal (due solely to Ca2+ released from cortical stores) recovered with t1/2 65 min. When ASs were analyzed in situ by X-ray microanalysis after different trigger times (+Ca2+o), t1/2 for store refilling was similar, 60 min. These values are similar to previously measured 45Ca2+-uptake by isolated ASs. In sum we find, (i) exogenous Ca2+ increases exocytosis/membrane fusion performance with EC50=0.7 μM, (ii) Ca2+-signaling in this system is not sensitive to actin-reactive drugs, and (iii) refilling of these cortical calcium stores goes on over hours and thus is much slower than expected.</dcterms:abstract>
    <dc:creator>Mohamed, Ihab K.</dc:creator>
    <dc:creator>Husser, Marc R.</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:25Z</dc:date>
    <dc:creator>Hentschel, C.</dc:creator>
    <dc:creator>Hentschel, Joachim</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Cell Calcium ; 34 (2003), 1. - S. 87-96</dcterms:bibliographicCitation>
    <dc:creator>Plattner, Helmut</dc:creator>
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