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The DEG15 Serine Protease Cleaves Peroxisomal Targeting Signal 2-Containing Proteins in Arabidopsis

The DEG15 Serine Protease Cleaves Peroxisomal Targeting Signal 2-Containing Proteins in Arabidopsis

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SCHUHMANN, Holger, Pitter Florian HUESGEN, Christine GIETL, Iwona ADAMSKA, 2008. The DEG15 Serine Protease Cleaves Peroxisomal Targeting Signal 2-Containing Proteins in Arabidopsis. In: Plant Physiology. 148(4), pp. 1847-1856. ISSN 0032-0889. eISSN 1532-2548. Available under: doi: 10.1104/pp.108.125377

@article{Schuhmann2008DEG15-8467, title={The DEG15 Serine Protease Cleaves Peroxisomal Targeting Signal 2-Containing Proteins in Arabidopsis}, year={2008}, doi={10.1104/pp.108.125377}, number={4}, volume={148}, issn={0032-0889}, journal={Plant Physiology}, pages={1847--1856}, author={Schuhmann, Holger and Huesgen, Pitter Florian and Gietl, Christine and Adamska, Iwona} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8467"> <dc:contributor>Adamska, Iwona</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:53Z</dcterms:available> <dcterms:abstract xml:lang="eng">Two distinct peroxisomal targeting signals (PTSs), the C-terminal PTS1 and the N-terminal PTS2, are defined. Processing of the PTS2 on protein import is conserved in higher eukaryotes. Recently, candidates for the responsible processing protease were identified from plants (DEG15) and mammals (TYSND1). We demonstrate that plants lacking DEG15 show an expressed phenotype potentially linked to reduced ß-oxidation, indicating the impact of protein processing on peroxisomal functions in higher eukaryotes. Mutational analysis of Arabidopsis (Arabidopsis thaliana) DEG15 revealed that conserved histidine, aspartic acid, and serine residues are essential for the proteolytic activity of this enzyme in vitro. This indicates that DEG15 and related enzymes are trypsin-like serine endopeptidases. Deletion of a plant-specific stretch present in the protease domain diminished, but did not abolish, the proteolytic activity of DEG15 against the PTS2-containing glyoxysomal malate dehydrogenase. Fluorescence microscopy showed that a DEG15-green fluorescent protein fusion construct is targeted to peroxisomes in planta. In vivo studies with isolated homozygous deg15 knockout mutants and complemented mutant lines suggest that this enzyme mediates general processing of PTS2-containing proteins.</dcterms:abstract> <dc:contributor>Gietl, Christine</dc:contributor> <dc:creator>Adamska, Iwona</dc:creator> <dc:contributor>Schuhmann, Holger</dc:contributor> <dc:creator>Huesgen, Pitter Florian</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8467"/> <dcterms:rights rdf:resource="https://kops.uni-konstanz.de/page/termsofuse"/> <dcterms:bibliographicCitation>First publ. in: Plant Physiology 148 (2008), 4, pp. 1847-1856</dcterms:bibliographicCitation> <dc:rights>terms-of-use</dc:rights> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:title>The DEG15 Serine Protease Cleaves Peroxisomal Targeting Signal 2-Containing Proteins in Arabidopsis</dcterms:title> <dcterms:issued>2008</dcterms:issued> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:language>eng</dc:language> <dc:creator>Schuhmann, Holger</dc:creator> <dc:creator>Gietl, Christine</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:format>application/pdf</dc:format> <dc:contributor>Huesgen, Pitter Florian</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:53Z</dc:date> </rdf:Description> </rdf:RDF>

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