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The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown

The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown

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GÖHRING, Frank, Birgit L. SCHWAB, Pierluigi NICOTERA, Marcel LEIST, Frank O. FACKELMAYER, 1997. The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown. In: The EMBO Journal. 16(24), pp. 7361-7371. ISSN 0261-4189. eISSN 1460-2075

@article{Gohring1997novel-8454, title={The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown}, year={1997}, doi={10.1093/emboj/16.24.7361}, number={24}, volume={16}, issn={0261-4189}, journal={The EMBO Journal}, pages={7361--7371}, author={Göhring, Frank and Schwab, Birgit L. and Nicotera, Pierluigi and Leist, Marcel and Fackelmayer, Frank O.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8454"> <dcterms:bibliographicCitation>First publ. in: The EMBO Journal ; 16 (1997), 24. - pp. 7361-7371</dcterms:bibliographicCitation> <dc:contributor>Nicotera, Pierluigi</dc:contributor> <dc:format>application/pdf</dc:format> <dcterms:title>The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown</dcterms:title> <dc:contributor>Fackelmayer, Frank O.</dc:contributor> <dcterms:issued>1997</dcterms:issued> <dc:creator>Nicotera, Pierluigi</dc:creator> <dc:rights>deposit-license</dc:rights> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8454"/> <dc:creator>Schwab, Birgit L.</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:48Z</dc:date> <dcterms:abstract xml:lang="eng">The scaffold attachment factor A (SAF-A) is an abundant component of the nuclear scaffold and of chromatin, and also occurs in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. Evidence from previous experiments had suggested that SAF-A most likely has at least two different functions, being involved both in nuclear architecture and RNA metabolism. We now show that the protein has a novel scaffold-associated region (SAR)-specifi bipartite DNA-binding domain which is independent from the previously identifie RNA-binding domain, the RGG box. During apoptosis, but not during necrosis, SAF-A is cleaved in a caspasedependent way. Cleavage occurs within the bipartite DNA-binding domain, resulting in a loss of DNAbinding activity and a concomitant detachment of SAF-A from nuclear structural sites. On the other hand, cleavage does not compromise the association of SAF-A with hnRNP complexes, indicating that the function of SAF-A in RNA metabolism is not affected in apoptosis. Our results suggest that detachment of SAF-A from SARs, caused by apoptotic proteolysis of its DNA-binding domain, is linked to the formation of oligonucleosomal-sized DNA fragments and could therefore contribute to nuclear breakdown in apoptotic cells.</dcterms:abstract> <dc:creator>Fackelmayer, Frank O.</dc:creator> <dc:creator>Göhring, Frank</dc:creator> <dc:language>eng</dc:language> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:48Z</dcterms:available> <dc:contributor>Göhring, Frank</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:contributor>Leist, Marcel</dc:contributor> <dc:contributor>Schwab, Birgit L.</dc:contributor> <dc:creator>Leist, Marcel</dc:creator> </rdf:Description> </rdf:RDF>

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