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Immunolocalisation of PrPSc in scrapie-infected N2a mouse neuroblastoma cells by light and electron microscopy

Immunolocalisation of PrPSc in scrapie-infected N2a mouse neuroblastoma cells by light and electron microscopy

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Prüfsumme: MD5:e264cd86159f7ad397df590266f1d194

VEITH, Nathalie Monika, Helmut PLATTNER, Claudia STÜRMER, Walter J. SCHULZ-SCHAEFFER, Alexander BÜRKLE, 2009. Immunolocalisation of PrPSc in scrapie-infected N2a mouse neuroblastoma cells by light and electron microscopy. In: European Journal of Cell Biology. 88(1), pp. 45-63. ISSN 0171-9335. eISSN 1618-1298

@article{Veith2009Immun-8445, title={Immunolocalisation of PrPSc in scrapie-infected N2a mouse neuroblastoma cells by light and electron microscopy}, year={2009}, doi={10.1016/j.ejcb.2008.08.001}, number={1}, volume={88}, issn={0171-9335}, journal={European Journal of Cell Biology}, pages={45--63}, author={Veith, Nathalie Monika and Plattner, Helmut and Stürmer, Claudia and Schulz-Schaeffer, Walter J. and Bürkle, Alexander} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8445"> <dc:creator>Schulz-Schaeffer, Walter J.</dc:creator> <dcterms:issued>2009</dcterms:issued> <dc:contributor>Plattner, Helmut</dc:contributor> <dc:contributor>Bürkle, Alexander</dc:contributor> <dc:creator>Plattner, Helmut</dc:creator> <dc:contributor>Schulz-Schaeffer, Walter J.</dc:contributor> <dcterms:abstract xml:lang="eng">The causative agent of transmissible spongiform encephalopathies (TSE) is PrPSc, an infectious, misfolded isoform of the cellular prion protein (PrPC). The localisation and trafficking of PrPSc and sites of conversion from PrPC to PrPSc are under debate, particularly since most published work did not discriminate between PrPC and PrPSc. Here we describe the localisation of PrPC and PrPSc in a scrapie-infected neuroblastoma cell line, ScN2a, by light and electron microscopic immunolocalisation. After eliminating PrPC with proteinase K, PrPSc was detected at the plasma membrane, endocytosed via clathrin-coated pits and delivered to early endosomes. Finally, PrPSc was detected in late endosomes/lysosomes. As we detected PrPSc at the cell surface, in early endosomes and in late endosomes/lysosomes, i.e. locations where PrPC is also present, our data imply that the conversion process could take place at the plasma membrane and/or along the endocytic pathway. Finally, we observed the release of PrPC/PrPSc via exocytotic pathways, i.e. via exosomes and as an opaque electron-dense mass which may represent a mechanism of intercellular spreading of infectious prions.</dcterms:abstract> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8445"/> <dc:rights>deposit-license</dc:rights> <dcterms:title>Immunolocalisation of PrPSc in scrapie-infected N2a mouse neuroblastoma cells by light and electron microscopy</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:44Z</dcterms:available> <dc:creator>Veith, Nathalie Monika</dc:creator> <dc:language>eng</dc:language> <dc:format>application/pdf</dc:format> <dc:contributor>Veith, Nathalie Monika</dc:contributor> <dc:creator>Stürmer, Claudia</dc:creator> <dcterms:bibliographicCitation>First publ. in: European Journal of Cell Biology 88 (2009), 1, pp. 45 63</dcterms:bibliographicCitation> <dc:creator>Bürkle, Alexander</dc:creator> <dc:contributor>Stürmer, Claudia</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:44Z</dc:date> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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