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Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy

Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy

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Prüfsumme: MD5:0ae1ca70a79571c3e0a51ecaba27d398

KLEBENSBERGER, Janosch, Karin LAUTENSCHLAGER, Daniel BRESSLER, Jost WINGENDER, Bodo PHILIPP, 2007. Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy. In: Environmental Microbiology. 9(9), pp. 2247-2259. ISSN 1462-2912. eISSN 1462-2920

@article{Klebensberger2007Deter-8417, title={Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy}, year={2007}, doi={10.1111/j.1462-2920.2007.01339.x}, number={9}, volume={9}, issn={1462-2912}, journal={Environmental Microbiology}, pages={2247--2259}, author={Klebensberger, Janosch and Lautenschlager, Karin and Bressler, Daniel and Wingender, Jost and Philipp, Bodo} }

Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy Bressler, Daniel Philipp, Bodo First publ. in: Environmental Microbiology 9 (2007), 9, pp. 2247-2259 Wingender, Jost Klebensberger, Janosch Philipp, Bodo 2011-03-24T17:43:26Z deposit-license Bressler, Daniel eng Wingender, Jost During growth of Pseudomonas aeruginosa strain PAO1 with the toxic detergent SDS, a part of the population actively formed macroscopic cell aggregates while the other part grew as freely suspended cells. The physiological function of aggregation for growth with SDS was investigated. Three mutants growing with SDS without aggregation were isolated: the spontaneous mutant strain N and two mutants with transposon insertions in the psl operon for exopolysaccharide synthesis. SDS-induced aggregation in strain N but not in a pslJ mutant was restored by complementation with two genes encoding diguanylate cyclases responsible for synthesis of cyclic-di-guanosine monophosphate (c-di-GMP). By expressing a c-di-GMP-specific phosphodiesterase SDS-induced aggregation of strain PAO1 was reduced.<br />Upon exposure to SDS in the presence of the uncoupler carbonyl cyanide chlorophenylhydrazone, the aggregating strains had ca. 500-fold higher survival rates than the non-aggregating strains. Co-incubation experiments revealed that strain N could integrate into aggregates of strain PAO1 and thereby increase its survival rate more than 1000-fold.<br />These results showed that SDS-induced aggregation involved c-di-GMP signalling with the psl operon as a possible target. Cell aggregation could serve as a pre-adaptive strategy ensuring survival and growth of P. aeruginosa populations in environments with multiple toxic chemicals. 2007 application/pdf 2011-03-24T17:43:26Z Lautenschlager, Karin Klebensberger, Janosch Lautenschlager, Karin

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