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Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+ T cells

Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+ T cells

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LESNIEWSKI, Mathew L., Peter HAVIERNIK, Patrick R. WEITZEL, Suzanne KADEREIT, Margaret M. KOZIK, Laura R. FANNING, Yu-Chung YANG, Yael HEGERFELDT, Marcie R. FINNEY, Mariuz Z. RATAJCZAK, Nicholas J. GRECO, Pascale PAUL, Jaroslaw MACIEJEWSKI, Mary J. LAUGHLIN, 2008. Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+ T cells. In: Leukemia. 22(12), pp. 2201-2207. ISSN 0887-6924. eISSN 1476-5551

@article{Lesniewski2008Regul-8403, title={Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+ T cells}, year={2008}, doi={10.1038/leu.2008.234}, number={12}, volume={22}, issn={0887-6924}, journal={Leukemia}, pages={2201--2207}, author={Lesniewski, Mathew L. and Haviernik, Peter and Weitzel, Patrick R. and Kadereit, Suzanne and Kozik, Margaret M. and Fanning, Laura R. and Yang, Yu-Chung and Hegerfeldt, Yael and Finney, Marcie R. and Ratajczak, Mariuz Z. and Greco, Nicholas J. and Paul, Pascale and Maciejewski, Jaroslaw and Laughlin, Mary J.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8403"> <dc:contributor>Paul, Pascale</dc:contributor> <dc:creator>Finney, Marcie R.</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8403"/> <dc:contributor>Ratajczak, Mariuz Z.</dc:contributor> <dc:contributor>Lesniewski, Mathew L.</dc:contributor> <dc:creator>Lesniewski, Mathew L.</dc:creator> <dc:creator>Paul, Pascale</dc:creator> <dcterms:bibliographicCitation>First publ. in: Leukemia 22 (2008), 12, pp. 2201 2207</dcterms:bibliographicCitation> <dc:creator>Kadereit, Suzanne</dc:creator> <dc:creator>Ratajczak, Mariuz Z.</dc:creator> <dc:creator>Laughlin, Mary J.</dc:creator> <dc:contributor>Hegerfeldt, Yael</dc:contributor> <dc:creator>Weitzel, Patrick R.</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Yang, Yu-Chung</dc:contributor> <dc:creator>Yang, Yu-Chung</dc:creator> <dc:contributor>Fanning, Laura R.</dc:contributor> <dc:creator>Greco, Nicholas J.</dc:creator> <dc:creator>Maciejewski, Jaroslaw</dc:creator> <dcterms:title>Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+ T cells</dcterms:title> <dc:creator>Kozik, Margaret M.</dc:creator> <dcterms:abstract xml:lang="eng">On activation, umbilical cord blood (UCB) CD4+ T cells demonstrate reduced expression of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), where as maintaining equivalent interleukin-2 (IL-2) levels, as compared with adult peripheral blood (PB) CD4þ T cells. Nuclear factor of activated T cells (NFAT1) protein, a transcription factor known to regulate the expression of IL-2, TNF-α and IFN-γ, is reduced in resting and activated UCB CD4+ T cells. In contrast, expression of Broad-complex-Tramtrack-Bric-a-Brac and Cap n collar homology 1 bZip transcription factor 2 (BACH2) was shown by gene array analyses to be increased in UCB CD4+ T cells and was validated by qRT-PCR. Using chromatin immunoprecipitation, BACH2 was shown binding to the human IL-2 proximal promoter. Knockdown experiments of BACH2 by transient transfection of UCB CD4+ T cells with BACH2 siRNA resulted in significant reductions in stimulated IL-2 production. Decreased IL-2 gene transcription in UCB CD4+ T cells transfected with BACH2 siRNA was confirmed by a human IL-2 luciferase assay. In summary, BACH2 maintains IL-2 expression in UCB CD4þ T cells at levels equivalent to adult PB CD4+ T cells despite reduced NFAT1 protein expression. Thus, BACH2 expression is necessary to maintain IL-2 production when NFAT1 protein is reduced, potentially impacting UCB graft CD4+ T-cell allogeneic responses.</dcterms:abstract> <dc:contributor>Finney, Marcie R.</dc:contributor> <dc:format>application/pdf</dc:format> <dc:creator>Hegerfeldt, Yael</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:20Z</dc:date> <dc:contributor>Laughlin, Mary J.</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:contributor>Haviernik, Peter</dc:contributor> <dc:contributor>Maciejewski, Jaroslaw</dc:contributor> <dc:contributor>Kadereit, Suzanne</dc:contributor> <dc:contributor>Weitzel, Patrick R.</dc:contributor> <dc:creator>Haviernik, Peter</dc:creator> <dc:contributor>Greco, Nicholas J.</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:20Z</dcterms:available> <dcterms:issued>2008</dcterms:issued> <dc:rights>deposit-license</dc:rights> <dc:contributor>Kozik, Margaret M.</dc:contributor> <dc:creator>Fanning, Laura R.</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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