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Molecular components and organelles involved in calcium-mediated signal-transduction in Paramecium

Molecular components and organelles involved in calcium-mediated signal-transduction in Paramecium

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SEHRING, Ivonne Margarete, 2006. Molecular components and organelles involved in calcium-mediated signal-transduction in Paramecium

@phdthesis{Sehring2006Molec-8378, title={Molecular components and organelles involved in calcium-mediated signal-transduction in Paramecium}, year={2006}, author={Sehring, Ivonne Margarete}, address={Konstanz}, school={Universität Konstanz} }

This thesis works on two themes. In the first part of the work, calcium dynamics upon triggering of Paramecium cells with GTP were performed using fluorochrome analysis. The results are presented in Sehring et al. (2004). Paramecium reacts upon GTP response with periodic back- and forward swimming. A reversion of the cilia stroke is achieved by high levels of calcium in cilia. On electrophysiology level, this behavioural response is accompanied with depolarisations of the membrane potential. In this work, different components which were proposed to be involved in the intracellular signaling (according to the model of Wessenberg et al. 1997; Figure 6) were investigated. We showed that calcium from the external medium as well as from intracellular stores is involved in signal transduction. In contrast to the model, neither the voltage-dependent ciliary calcium channels nor the cortical calcium stores, the alveolar sacs, participate in the reaction. The identity of the influx channels is still unknown. As intracellular store the ER could take part. Both needs further investigations.<br />As could be shown, the alveolarsacs are not involved in the reaction upon triggering with GTP. But as cortical calcium stores, they play a major role in exocytosis. An open question is their positioning and structural connection to the plasma membrane. A potential candidate is actin, as it was shown to be involved in calcium signaling in other systems. Thus, in the second part of this thesis, actin in Paramecium was investigated. At the beginning, a polyclonal antibody against actin1-1 was available which was used for immunohistological studies. The data obtained were published in Kissmehl et al. (2004). With the ongoing genome project, all genes coding for actin, actin-related proteins or actin-like proteins in Paramecium could be identified. A characterization of the paralogs on nucleotide and amino acid level is presented in the manuscript Kissmehl et al. (2006). Some of the genes were analyzed in detail, especially their localization using green-fluorescent proteins and/or specific antibodies, and their functional significance using RNAi (RNA interference). The obtained data revealed a specific targeting for some of the paralogs with a consistent differentiation function. The results are presented in the manuscript Sehring et al. (2006). Molecular components and organelles involved in calcium-mediated signal-transduction in Paramecium 2006 Sehring, Ivonne Margarete Sehring, Ivonne Margarete deposit-license Zusammenwirken molekularer Komponenten und Organelle bei der Calcium-vermittelten Signaltransduktion in Paramecium 2011-03-24T17:43:08Z eng 2011-03-24T17:43:08Z application/pdf

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

DissertationIMSehring2.pdf 66
DissertationIMSehring1.pdf 48

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